早产兔生发基质-脑室内出血模型的建立及神经行为学评价

摘要

Objective To establish the preterm rabbit of germinal matrix hemorrhage-intra-ventricular hemorrhage ( GMH-IVH) . Methods 29 premature rabbits were given birth by cesarean section. Then the premature rabbits were randomly divided into two groups;experimental group (n=18) and control group (n=18). Three hours later, the premature rabbits in experimental group were given intra-peritoneal injection of 50% glycerol (10 ml/kg), the preterm rabbits in control group were given intra-peritoneal injection with the same dose of saline. Both groups received the Cranial ultrasound scan check at 24 h after birth. Premature rabbits in experimental group undergoing GMH-IVH was called GMH-IVH model group, the control group not undergoing GMH-IVH in premature rabbits was called normal control group. Presence of GMH-IVH and neurobehavioral assess-ment were checked. Results In experimental group, the incidence rate of GMH-IVH was 83. 33%(15/18) and the incidence rate of GMH-IVH was 11. 11% (2/18) in control group. The incidence rate of GMH-IVH in experimental group was significant-ly higher than that of control group (P0. 05). Conclusion We have established animal models. The changes in this model are similar with clinical behavior characteristics of GMH-IVH in preterm children.%目的：建立早产兔生发基质-脑室内出血( germinal matrix hemorrhage-intra-ventricular hemorrhage, GMH-IVH)的动物模型,并进行神经行为学评价。方法将妊娠29 d兔进行剖宫产,迅速取出幼兔,随机分为实验组及对照组,每组各18只。生后3 h实验组予50%丙三醇腹腔注射,对照组接受等剂量0.9%氯化钠注射液腹腔注射,均于出生后24 h接受头颅彩色多普勒超声检查。实验组发生GMH-IVH的早产兔为GMH-IVH模型组,对照组未发生GMH-IVH的早产兔为正常对照组,观察GMH-IVH 的发生率并进行神经行为学评价。结果早产兔GMH-IVH的发生率实验组、对照组分别为83.33%(15/18)、11.11%(2/18),两组比较差异有统计学意义(P0．05)。结论早产兔予腹腔注射丙三醇可建立GMH-IVH动物模型,此模型符合早产儿GMH-IVH的临床行为改变。