不同浓度羟考酮对结直肠癌细胞生物学行为的影响

摘要

Objective To investigate the effect of oxycodone on the biological behavior of colorectal cancer ( CRC) cells. Methods SW620 CRC cells were inoculated and incubated for 24 h, followed by treatment with oxy-codone at 0, 10, 20 and 40 μg/ml for 24 h, and then incubation with CCK-8 kit. The microplate reader was used to measure optical density at a wavelength of 490 nm in different concentration groups, to compare the difference in in-hibition rate of cell proliferation. The effects of oxycodone at different concentrations on apoptosis and migration of SW620 cells were detected by flow cytometry FITC-Annexin V/PI and the scratch test respectively. In addition, the impact of different concentrations of oxycodone on the VEGF expression of SW620 cells was detected by ELISA. Re-sults As compared with the control group ( non-oxycodone treatment group) , oxycodone inhibited the proliferation of apoptosis of SW620 cell in different concentrations of oxycodone treatment groups. There were significant differ-ences in apoptosis of SW620 cells in 20 and 40 μg/ml groups, as compared with that in control group ( t＝12. 64, P＝0. 019; t ＝13. 94, P ＝0. 016 ) . The migration distance of SW620 cells was significantly shorter in 20 and 40 μg/ml groups than in control group(t＝11. 29,P＝0. 036;t＝12. 05,P＝0. 009). The expression levels of VEGF in SW620 cells were significantly lower in 20 and 40 μg/ml groups than in control group(t＝12. 62,P＝0. 031; t＝13. 61,P＝0. 013). Conclusion Oxycodone can suppress the proliferation and migration of CRC cells in a dose-de-pendent manner, inhibit VEGF production and secretion, and induce apoptosis of cells.%目的 探讨羟考酮对结直肠癌( colorectal cancer, CRC)细胞生物学行为的影响.方法 选择人CRC SW620细胞接种并孵育24 h后,分别以0、10、20和40 μg/ml羟考酮处理24 h,然后使用CCK-8试剂盒孵育后,酶标仪板在490 nm波长下测量不同浓度组的光密度值,比较细胞增殖抑制率差异;采用流式细胞术FITC-Annexin V/PI检测不同浓度羟考酮对SW620 细胞凋亡的影响;行划痕实验检测不同浓度羟考酮对SW620细胞迁移的影响;采用酶联免疫吸附法检测不同浓度羟考酮对 SW620 细胞中血管内皮生长因子(VEGF)的表达.结果 相较于对照组(未经羟考酮处理组),10、20、40 μg/ml羟考酮处理组SW620细胞增殖能力受到显著抑制. 20和40 μg/ml羟考酮处理组SW620 细胞凋亡率与对照组比较差异有统计学意义(t＝12. 64,P＝0. 019;t＝13. 94,P＝0. 016). 20和40 μg/ml羟考酮处理组SW620细胞迁移距离比例显著低于对照组(t＝11. 29,P＝0. 036;t＝12. 05,P＝0. 009). 20和40 μg/ml羟考酮处理组SW620细胞VEGF表达水平显著低于对照组(t＝12. 62,P＝0. 031;t＝13. 61,P＝0. 013).结论 羟考酮能以剂量依赖的方式对CRC细胞的增殖和迁移起到抑制作用,抑制VEGF的产生和分泌,同时还能诱导细胞的凋亡.