基质金属蛋白酶11和组织蛋白酶D在胃腺癌组织中的表达及其与预后的关系

摘要

目的 探讨胃腺癌组织中基质金属蛋白酶11( matrix metalloprotein-11,MMP-11)、组织蛋白酶D(cathepsin D,Cath-D)的表达及与预后的关系.方法 收集华北理工大学附属医院2010年1月至2013年12月胃腺癌患者的临床资料及术后标本共95例作为观察组,收集上述术后标本中距肿瘤边缘＞3 cm的非肿瘤性的胃黏膜组织70例作为对照组,采用免疫组化法检测MMP-11和Cath-D的表达.结果 MMP-11在观察组和对照组中表达的阳性率分别为51. 6%(49/95)、5. 7%(4/70),两组比较差异有统计学意义(χ2＝38. 884,P＜0. 05). Cath-D在观察组和对照组中表达的阳性率分别为73. 7%(70/95)、28. 6%(20/70),两组比较差异有统计学意义(χ2＝33. 082,P＜0. 05). MMP-11表达的阳性率与肿瘤的淋巴结转移和脉管浸润有关(χ2 值分别为7. 193、15. 566,P均＜0. 05);Cath-D表达的阳性率与肿瘤最大径、淋巴结转移、脉管浸润和增殖指数有关( χ2 值分别为7. 431、5. 654、6. 569、6. 801,P均＜0. 05).相关性分析显示癌组织中MMP-11和Cath-D呈正相关(r＝0. 46,P＜0. 05).经生存分析显示, MMP-11 和 Cath-D 高表达的患者预后较差( P 均＜0. 05).结论 胃腺癌组织中MMP-11和Cath-D呈高表达且具有协同作用,MMP-11和Cath-D的表达与预后有关.%Objective To detect the expressions of Matrix Metalloprotein-11 ( MMP-11 ) and Cathepsin-D(Cath-D),and investigate their relationship and prognostic significance. Methods The study included 95 cases′ clinical date and postoperative specimens of gastric adenocarcinoma ( North China University of Science and Technology Affiliated Hospital,2010. 01-2013. 12) as observation group,70 cases of normal gastric tissue(from observation group) as control group. Expressions of MMP-11 and Cath-D were detected by IHC methods in two groups. Results The positive rate of MMP-11 was 51. 6%( 49/95) in observation group,5. 7%(4/70)in control group(χ2＝38. 884,P＜0. 05). The positive rate of Cath-D was 73. 7%(70/95) in observation group,28. 6%(20/70) in control group(χ2＝33. 082,P＜0. 05). The positive rate of MMP-11 was correlated with metastasis and vascular invasion(χ2＝7. 193、15. 566,P＜0. 05). The positive rate of Cath-D was correlated with maximal diameter,lymph node metastasis,vascular invasion and proliferation index(χ2＝7.431、5.654、6.569、6.801,P＜0.05).There was positive relationship between MMP-11 and Cath-D in observation group(r＝0. 46,P＜0. 05). The expressions of MMP-11 and Cath-D were correlated with prognosis in gastric adenocarcinoma ( P＜0. 05) . Conclusion The higher expressions and synergistic effect of MMP-11 and Cath-D may promote the occurrence and development in gastric adenocarcinoma. The joint detection of MMP-11 and Cath-D may be helpful to predict the prognosis of gastric adenocarcinoma.