THE BINDING BEHAVIOUR OF IMMOBILISED LOW MOLECULAR WEIGHT LIGAND WITH PEPTIDES IN BIOSENSOR-BASED SYSTEM

摘要

l introductionImmobilised llletal ion amthe chromatography(IMAC) is one of the importallt methods used inProteill separahon and Purificahon. The bonily Ofnattirally occtlrrillg Proteins for IMAC ligandsbasically relates to tile 11Umber of ltishdine residuesand the tOPology of these residues on the protein'ssLuice[1-21. However the occuzrence Of s~eaccessible histidine in globular proteins is rare,atypically amounting to ~ 2% of tile total alinno acidcolltellt[3-4]. The application of IMAC for thepurificatio...