PCR检测HBV DNA与HBV血清标志物常见模式和Pre-S2相互关系研究

摘要

目的　探讨乙型肝炎病毒(HBV)DNA与六种常见HBV血清免疫学标志物模式、前S2蛋白抗原(Pre-S2)之间的相互关系及其临床检测意义。方法　采用PCR法对HBV DNA、ELISA法对HBV血清免疫学标志物、Pre-S2同时进行检测。结果　1 484例六种常见模式HBV DNA阳性率依次为89.8%>55.6%>21.8%>7.5%>7.1%>6.8%，即模式(1)>(3)>(2)>(5)>(6)>(4)；而HBV血清免疫学标志阳性例数依次为(2)>(1)>(3)>(5)>(4)>(6)。(2)、(3)种模式HBV DNA与Pre-S2阳性率比较P<0.01，其余(1)、(4)～(6)种模式HBV DNA与Pre-S2阳性检出率无显著性差异(P>0.05)。结论　HBV血清免疫学标志物、Pre－S2、HBV DNA的检测，三者缺一不可，ELISA法检测HBV血清免疫学标志物、Pre－S2，只是HBV的表型指标，提供HBV感染的间接证据。而PCR－HBV DNA的检测是HBV感染与否的直接证据。因此它们各自有其独特的临床检测意义。%Objective　To investigate the clinical significance and relationship among Pre-S2、6 common types of HBV serum immune markers(HBV-M)and HBV-DNA.Methods　Using PCR to detect HBV DNA and ELISA technique to detect Pre-S2,6 kinds of HBV-M in the meantime.Results　Positive infection rates of HBV DNA in 6 common types of the 1484 cases were 89.8%>55.6%>21.8%>7.5%>7.1%>6.8% respectively,namely the model (1)>(3)>(2)>(5)>(6)>(4);but the positive cases of HBV serum immune markers were in the order (2)>(1)>(3)>(4)>(5)>(6).The positive rates were significant difference between Pre-S2 and HBV DNA in (2)、(3) types (P<0.01) and no significant difference in other types(P>0.05).Conclusion　It is necessary to detect Pre-S2,HBV DNA and HBVserum immune markers,but HBV serum immune makers and Pre-S2 detected by ELISA only external indication and indirect evidence of HBV infection.While the detection of HBV DNA by PCR was direct evidence of HBV infection.Therefore they have their unique clinicl significance.