采用新鲜全血比对控制ADVIA2120五分类血液分析仪质量的方法探讨

摘要

目的 探讨在没有质控品的情况下用新鲜全血对ADVIA2120血液分析仪进行室内质量控制的效果.方法 每天用新鲜全血标本,在经溯源至参考方法的配套校准物校准的迈瑞BC-3000 Plus三分群血液分析仪及经其定值的新鲜血校准的ADVIA2120五分类血液分析仪上测定,比对两台仪器所测主要参数白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)、血小板(PLT)、红细胞比容(HCT)、平均红细胞体积(MCV),以此作为ADVIA2120五分类血液分析仪的室内质量控制.结果 两台仪器PLT平均偏差均小于10%,其他参数平均偏差小于5%.均未超过美国CLIA′88能力比对检验的分析质量要求推荐的允许误差范围,但RBC及PLT有几次超出允许误差范围,按失控处理.结论 同一个实验室内不同血液分析仪用新鲜血每日比对是控制质量的可行方法.%Objective To observe the effect of quality control for hematology analyzers with fresh whole blood without the control material.Methods Fresh whole blood samples were tested daily on the BC3000 Plus analyzer which was calibrated with cali brate material recommend by manufacturer and on the ADVIA2120 analyzer,which was calibrated by the fresh whole blood.Results of such primary parameters as WBC,RBC, HGB, HCT, MCV and PldT were compared to establish the quality control method for the ADVIA 2120. Results The average differences of all parameters between the two analyzers were smaller than 5％ , except for PLT, which was smaller than 10 ％ .But the RBC and PLT were out of control, which were beyond the range of allowable error.Conclusion It was a recommendable method that laboratories carry out quality control with fresh whole blood daily comparison be tween different hematology analyzers.