3-氨基苯甲酰胺影响糖尿病大鼠晶状体上皮细胞NF-κB表达的免疫组化研究

摘要

Objective To observe the effection of poly ADP ribose polymerase (poly ADP ribose polymerase,PARP) inhibitor 3 aminobenzamide (3 AB) on the expression of nuclear factor Kappa B (nuclear factor kappa B,NF κB) in the early diabetic mellitus(diabetes mellitus,DM) rats modal. Methods The rats were intervented by the intraperitional injection of l％ Stretozocin at adoze of 50 mg/kg. The rats were divided into N group,DM group,DM+P group. Starting from the third day after modeling,DM+ P group were given 3 AB 30 mg/kg per day orally. At the 2nd,4th,8th week,the rats were sacrificed. Results The immunohisto chemistry results showed that the expression of NF κB p65 increased statistically in DM group than N group at the corresponding time points 2nd,4th,8th week;The expression of NF κB p65 in DM+P group was significantly increased than N group at the 2nd,4th,8th week. The expression of NF κB p65 increased statistically in DM group at the 2nd,4th,8th week compared with the DM+ P group. Conclusion PARP inhibitor 3 AB can inhibit the expression of NF κB on the lens epithelial cells of the early DM rats.%目的 观察聚腺苷二磷酸核糖聚合酶(PARP)抑制剂3-氨基苯甲酰胺(3-AB)对早期糖尿病(DM)大鼠模型晶状体上皮细胞中核因子κB(NF-κB)表达的影响.方法 采用一次性腹腔注射1%链脲佐菌素(STZ)50 mg/kg建立DM大鼠模型.成模后将大鼠随机分为正常对照组(N组)、糖尿病组(DM组)和干预组(DM+P组).自建立模型后第3天起,DM+P组每日给予3-AB 30 mg/kg灌胃,N组和DM组每天给予等体积0.9%生理盐水灌胃.分别于给药后2、4、8周处死各组大鼠,取出晶状体,免疫组化SP法检测晶状体上皮细胞中NF-κB P65的表达情况.结果 DM 2、4、8周组大鼠晶状体上皮细胞NF-κB P65表达较相应时间点的N组高;DM+P 2、4、8周组大鼠晶状体上皮细胞NF-κB P65表达较相应时间点的N组高;DM+P 2、4、8周组与相应时间点DM组比较,晶状体上皮细胞NF-κB P65表达降低.结论 PARP抑制剂3-AB可以抑制早期糖尿病大鼠晶状体上皮细胞中NF-κB的表达.