幽门螺杆菌感染、端粒酶活性与胃癌、癌前病变关系的研究

摘要

目的 探讨幽门螺杆菌(Hp)、端粒酶在胃癌及癌前病变中的作用及相互关系.方法 应用Warthin-Starry银染法、免疫组织化学SP法及核酸原位杂交技术分别检测66例胃黏膜肠化生、36例异型增生、50例胃癌及10例正常胃黏膜Hp感染情况、hTERT蛋白及hTERT mRNA的表达情况.结果 胃黏膜肠化生、异型增生及胃癌中Hp感染率、hTERT蛋白及hTERT mRNA阳性表达率均显著高于正常胃黏膜.93例Hp感染阳性组hTERT蛋白表达阳性率为88.17%,59例Hp感染阴性组hTERT蛋白表达阳性率为47.46%,两组比较差异有统计学意义(P<0.01).hTERT蛋白表达阳性组hTERT mRNA表达阳性率显著高于hTERT蛋白表达阴性组(P<0.01).结论 Hp、hTERT蛋白及hTERT mRNA与癌前病变及胃癌发生有关,Hp感染可引起胃癌中hTERT蛋白表达水平的上升,从而激活端粒酶,这可能是Hp感染致胃癌的另一分子机制.%Objective To study the effect and correlation of infection of Helicobacter pylori and telomerase activity in gastric cancer , precancerous lesions.Methods Hp infection was detected by Warthin -Starry method , hTERT protein expressiun was examined by immunohistochemtcal technique SP,hTERT mRNA expression was examined by situ hybrtdization.Results Hp infection,the positive expression rate of hTERT protein and hTERT mRNA in intestnal metaplasia,epithelial dysplasia of stomach and gastric carcinomas were significantly higher than normal gastritis mucosa.The positive expression rate of hTERT protein in 93 cases of gastric mucosa lesion of positive Hp infection was 88.17％ , which was significantly higher than 47.46％ in 59 cases of negative Hp infection(P＜0.01).The positive expression rate of hTERT mRNA with positive hTERT protein was significantly higher than that with negative hTERT protein(P＜0.01).Conclusion Hp infection, hTERT protein and hTERT mRNA are associated with gastric carcinomas, Hp infection can result in increased expression of hTERT protein and then activate telomerase, which may be one of molecular mechanism by which Hp infection leads to gastric carcinoma.