摘要:
目的 评估液基细胞学检验(TCT)、人乳头状瘤病毒(HPV)、c-MYC、人染色体端粒酶RNA基因(hTERC)联合检测在宫颈癌筛查中的应用价值.方法 将本院妇科门诊接受检查的230例有性生活史的女性作为研究对象,分别进行TCT、HPV检测,并利用荧光原位杂交(FISH)法检测c-MYC、hTERC基因扩增情况,以病理组织学结果为金标准,分别比较四者单独及联合检测时对宫颈上皮内瘤变(CIN)Ⅱ/Ⅲ级及鳞状细胞癌(SCC)的敏感度、特异度、准确性.结果 230例筛查者中,TCT检测阳性124例,HPV阳性155例,c-MYC基因扩增118例,hTERC基因扩增128例.当TCT、HPV、c-MYC、hTERC单独使用时,HPV的敏感度最高(84.5%),c-MYC的特异度最高(97.6%);hTERC的准确性最高(85.2%).当两两组合时,TCT+ HPV与HPV+ hTERC的敏感度最高(均为89.2%),TCT+ hTERC的特异度最高(87.8%),c-MYC+ hTERC的准确性最高(86.5%).当三种指标任意配合使用时,TCT+ HPV+ hTERC的敏感度及准确性最高(98.6%、90.9%),TCT+ c-MYC+hTERC的特异度最高(79.3%).而四种指标联合使用时,敏感度为98.6%,特异度为72.0%,准确性为89.1%.结论 联合检查能提高宫颈病变筛查的敏感度和准确性,其中TCT+ HPV+ hTERC三者联合检查时诊断效率最佳,c-MYC基因检测具有最高的特异度.%Objective To investigate the application value of combination of thinprep cytologic test (TCT),human papilloma virus (HPV),c-MYC and human chromosome telomerase gene (hTERC) genes to screen cervical cancer.Methods A total of 230 cases of the study objects was detected with TCT and HP,respectively.Amplification of c-MYC and hTERC genes was tested with fluorescence in situ hybridization (FISH) method.The histopathological results were the gold standard,and the sensitivity,specificity and accuracy of cervical intraepithelial neoplasia (CIN) Ⅱ / Ⅲ and squamous cell carcinoma (SCC) were compared with the four combined detection.Results Of 230 screened patients,there were 124 cases of TCT positive,155 cases of HPV positive,c-MYC gene amplified in 118 cases,and hTERC gene amplified in 128 cases.When TCT,HPV,c-MYC,and hTERC were used alone,the highest sensitivity was HPV (84.5%),the highest specificity was c-MYC (97.6%),and the highest accuracy was hTERC (85.2%).When the three indexes were used in coordination with each other,the sensitivity and accuracy of TCT + HPV + hTERC were the highest (98.6% and 90.9%),and the specificity of TCT + c-MYC + hTERC was the highest (79.3%).When the four indexes were used together,the sensitivity was 98.6%,the specificity was 72.0%,and the accuracy was 89.1%.Conclusions Combined examination can improve the sensitivity and accuracy of screening cervical lesions,and the three combination of TCT + HPV + hTERC had the best effect,and c-MYC gene detection had the highest specificity.