Objective To establish the animal model of persistent tractive spinal cord injury in rabbits and to investigate the changes of tumor necrosis factor-α(TNF-α) in cerebrospinal fluid and its significance. Methods Totally 24 healthy rabbits were randomly divided into 4 groups:group A(control group),group B(sham-operated group,only installing internal fixation) and group C, DC stretching 25%,50% of transverse diameter of L1/2 articular surface and installing internal fixation. The level of TNF-α in cerebrospinal fluid was detected before operation and at 10 min,2 h,24 h,7d after operation. The hindlimb motor function in all groups was evaluated by using the Tarlov scoring before operation and at 8h, ld,3d,7d after operation. Results The level of TNF-α in the group C and D had obvious difference with the stretch distance increase in early postoperation. The contents of TNF-α in the group C and D were(134. 95 ± 19. 36)pg/mL and(155. 68 ± 10. 63) pg/mL at postoperative 10 min,and(158. 13 ± 14. 67)pg/mL and(181. 50±16. 98) pg/mL at postoperative 2h. Compared with before operation,postoperative 24 h,7 d in the same group and the same time points in the group A and B,differences showed statistical significance(P<0. 05). The lose of hindlimb motor function was correlated with the traction degrees of spinal cord. The motor function of hindlimb in the group A and B without traction had no change. The Tarlov scores of the hindlimb motor function in the group C and D were 5. 00 + 0. 00 before operation and 4. 17 ± 0. 41 and 3. 67 ± 0. 52 at postoperative 8 h. Compared with before operation,postoperative 1,3,7 d in the same group and the same time points in the group A and B,differences were statistically significant(P<0. 05,P<0. 01 respectively). Conclusion The change of the TNF-α level in cerebrospinal fluid in early spinal cord injury could be used a marker to evaluate the degree of spinal cord injury and the hindlimb motor function.%目的 建立兔持续性牵张脊髓损伤动物模型,探讨脑脊液中TNF-α的变化及其意义.方法 将24只健康兔子随机分为4组.A组为正常对照组;B组为假手术组,术中仅安装内固定;C、D组分别牵张L1/2小关节突叠合距离的25%、50%长度,安装内固定以维持牵张距离.测定各组术前及术后10 min、2 h、24 h、7 d脑脊液中TNF-α的含量;对各组术前、术后8 h、1 d、3 d、7 d进行后肢运动功能Tarlov评分.结果 C、D组脑脊液中TNF-α的含量在早期随着牵张距离的增加而升高,术后10 min脑脊液中TNF-α的含量分别为(134.95±19.36)、(155.68±10.63)pg/mL,术后2 h分别为(158.13±14.67)、(181.50±16.98)pg/mL,与同组术前、术后24 h、术后7 d及A、B组同时间比较,差异有统计学意义(P<0.05);后肢运动功能的丧失与脊髓牵张程度有相关性,A、B组牵张术后运动功能无变化,C、D组术前运动功能评分为(5.00±0.00)分,术后8 h分别为(4.17±0.41)、(3.67±0.52)分,与同组术前、术后1、3、7 d及A、B组同时间比较,差异有统计学意义(P<0.05).结论 脊髓损伤早期脑脊液中TNF-α含量的变化可作为评估脊髓损伤程度及后肢运动功能的一个指标.
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