肾动脉狭窄合并动脉粥样硬化大鼠模型的建立

摘要

Objective To explore an efficient method to establish a rat model of atherosclerosis combined with renal artery ster-nosis using surgery and vitamin D3 and high-fat diet. Methods One hundred and four healthy SD rats were randomly divided into 3 groups:control group,two-kidneys and one-clip(K2C1) group,one-kidneys and one-clip(K1C1) group, ten weeks after surgery and raise,BP,TC,TG,BUN,Scr in rat were detected, the sections of the aorta for HE and renal tissue were prepared for PAS dyeing observation. Results The level of BP,TC and BUN,Cr in surgery groups(K1C1 and K2C1) were obviously higher than that before model-constructing and in the control group(P<0. 05) , but TG was normal(P>0. 05). Obvious calcification, endometriosis thickening, smooth muscle cell proliferation and inflammatory cells infiltralion were,obvious in the surgery groups. The kidney had ischemia change in the surgery groups. Compared with the surgery groups,the control group had endometriosis thickening,smooth muscle cell proliferation and foam cells formation,but smaller. Conclusion The rat model of atherosclerosis combined with renal artery sternosis can be effectively established using surgery and vitamin D3 combined with high-fat diet.%目的 探讨手术联合维生素D3及高脂饮食建立大鼠肾动脉狭窄合并动脉粥样硬化(AS)模型的方法.方法 将健康雄性SD大鼠104只分为对照组、两肾一夹(K2C1)组及两肾均夹(K1C1)组,检测试验前及造模处理10周后血压(BP);血清中胆固醇(TC)、三酰甘油(TG)及尿素氮(BUN)、血肌酐(Scr).行主动脉HE染色及肾脏PAS染色观察.结果 造模完成后手术组(K1C1组和K2C1组)BP及TC、BUN、Scr与造模前及对照组比较均明显升高,差异有统计学意义(P<0.05);但K1C1组和K2C1组比较,上述指标差异无统计学意义(P>0.05).通过染色及镜下观察发现,手术组可见满意斑块形成,主动脉弓钙化明显,内膜显著增厚、平滑肌细胞增生及炎症细胞浸润,术侧肾脏发生缺血性改变;对照组则内膜增生凸向管腔,平滑肌细胞有增生,细胞排列紊乱,有少量泡沫细胞形成,但其变化较手术组为小.结论 通过手术及腹腔注射维生素D3 50万IU/Kg并联合高脂饲养,可有效建立肾动脉狭窄合并动脉粥样硬化动物模型.