大黄酸对糖尿病肾病大鼠足突细胞nephrin基因表达的影响

摘要

Objective To investigate rhein on podocyte nephrin gene expression and nephrin on glomerular in diabetic nephropa-thy (DN)rats .Methods DN rats mode were made by Intraperitoneal injection of streptozotocin (STZ) 60 mg/kg once .the DN model was divided into 2 groups:rhein treatment group (A) ,model group (B) ,and in addition ,set up a control group (C) .body weight ,kidney weight ,relative kidney weight ,24-hour urinary protein excretion ,serum creatinine ,blood urea nitrogen of rats were detected after 8 weeks of intervention .kidney pathological changes were observed by using light microscope ,electron microscope . immunohistochemical technique used to nephrin protein expression .Results Both the DN rats 24 -hour urinary protein excretion and podocyte nephrin gene expression of group A decreased significantly (P<0 .05) ,improvement in renal function .Conclusion Rhein on DN kidney has a protective effect ,which may be related to its podocyte nephrin gene expression.%目的：观察nephrin在糖尿病肾病（DN ）大鼠肾小球中的表达，研究大黄酸对nephrin表达的影响。方法用链脲佐菌素60 mg/kg一次性腹腔注射方式制作大鼠DN模型，将DN模型分为2组：大黄酸治疗组、模型组，另外设置模型对照组。各组均干预8周后，测量大鼠体质量、肾脏质量、相对肾质量、24 h尿蛋白定量、血清肌酐、尿素氮，利用光镜、电镜观察肾脏病理改变，应用免疫组化技术观察nephrin蛋白的表达情况。结果大黄酸治疗组24 h尿蛋白定量明显下降（P＜0．05），肾功能改善，足突细胞nephrin表达明显下降。结论大黄酸对DN肾脏有保护作用，而此种保护作用可能与其能够抑制足突细胞nephrin表达有关。