巨细胞病毒感染小鼠结肠中肠三叶因子的表达及更昔洛韦的干预作用

摘要

目的：观察肠三叶因子（ITF/TFF3）在鼠巨细胞病毒（MCMV）感染小鼠结肠中的表达及更昔洛韦的干预作用。方法将48只4周龄BALB/c小鼠，随机分为空白组、病毒组及更昔洛韦（GCV）组，每组8只。病毒组和GCV组分别腹腔接种致半数细胞感染量（TCID50＝105．31/mL）的MCMV病毒悬液100μL ，GCV组在接种MCMV 24 h后GCV 50 mg/kg ，每天1次，腹腔注射，连续14 d ，同时病毒组和空白组分别给予同等剂量的生理盐水作为对照。定量PCR检测肝脏、结肠MCMV-DNA ；采用实时定量逆转录-聚合酶链反应（RT-PCR）法检测ITF mRNA表达水平。结果病毒组小鼠明显出现食欲差、活动少；皮毛稀松、对刺激反应迟钝、生长迟缓、体质量不增等表现。病毒组肝脏、结肠组织有MCMV-DNA表达，GCV组亦有表达，但弱于病毒组。GCV组与病毒组相比ITF mRNA表达升高，差异有统计学意义（P＜0．05）；病毒组与空白组相比ITF mRNA表达水平升高，差异有统计学意义（P＜0．05）。结论 ITF是一类较新的肠黏膜保护因子，在MCMV感染后所导致的急性肠道炎症损伤过程中发挥着重要的保护作用。%Objective To observe the expression of ITF in colon of mice infected by mouse cytomegalovirus (MCMV) and the in-volvement of ganciclovir(GCV) .Methods Forty-eight BALB/c young mice were randomly divided into blank group ,virus group and GCV group ,each with 8 mice .Mice in virus group and GCV group received injection of 100 μL MCMV virus suspension (TCID50105 .31 /mL) ,and GCV group was given intraperitoneal injection of GCV once a day at the dose of 50 mg/kg from day 0 (24 hours after vaccination of virus ) ,for 14 days .At the same time the virus group and blank group were given the same dose of normal saline as controls .Murine cytomegalovirus loads in livers and colons were measured by PCR .The expression levels of ITF in mRNA in colon were detected by RT-PCR .Results After MCMV injection ,mice in virus group manifested aggressively apparent poor appetite ,less activity ,fur laxly ,unresponsiveness to stimuli ,growth retardation ,body weight not increased .All liver and colon tissue MCMV-DNA PCR electrophoresis of virus group had positive strip ,while the blank group did not .GCV group also showed less bright positive strip when compared with the virus group .Expression level of ITF mRNA was significant higher in GCV group than virus group ,there was statistically significant difference(P<0 .05) .Expression of ITF mRNA in virus group were higher than that in blank group ,there was statistical difference(P<0 .05) .Conclusion ITF is regarded as a fast reaction peptide in the course of mucosa impairments ,so ITF plays a protective role in delayed healing process after acute MCMV infection .