目的：探讨共染MIP-lα与B7-1诱导乳腺癌大鼠的免疫应答能力。方法采用SHZ-88乳腺癌细胞造模；每日观察SD大鼠接种乳腺癌细胞后有无肿瘤生长；将荷瘤大鼠随机分成对照组、M IP-1α组、B7-1组和联合组注射对应的转基因细胞进行治疗，分别在1～4周观察肿瘤体积变化和生存时间，收集大鼠外周血检测CD4＋、CD8＋百分比和CD4＋/CD8＋比值以及白细胞介素2（IL-2）、人γ干扰素（IFN-γ）水平并进行比较。结果对 SD大鼠分别接种SHZ-88、SHZ-88/MIP-1α、SHZ-88/B7-1及SHZ-88/MIP-1α＋B7-1细胞10 d后，各组的致瘤率分别为100％、20％、30％及0％；对照组、MIP-1α组、B7-1组和联合组注射对应的转基因细胞治疗后，联合组的肿瘤体积在1～4周均明显小于其他组，生存时间明显长于其他组，外周血CD4＋、CD8＋百分比和CD4＋/CD8＋比值以及IL-2、IFN-γ水平均明显高于其他组，以上比较差异均有统计学意义（ P＜0．05）。结论共染M IP-lα与B7-1，可诱导乳腺癌大鼠机体的免疫应答能力，起到抗肿瘤的作用。%Objective To investigate the ability of immune response of cotransfection of rat breast cancer cells with MIP-1αand B7-1 .Methods Tumor growth was observed every day after the SHZ-88 breast cancer cells were inoculated in the rats .The tumor-bearing rats were randomly divided into control group ,MIP-1αgroup ,B7-1 group and combination group ,which were injected with corresponding transgenic cells .The change of tumor volume in 1~4 weeks and the survival time were observed and compared .The peripheral blood was collected .CD4+ ,CD8+ percentage and CD4+ /CD8+ ratio and the levels of IL-2 and IFN-γof the peripheral blood were detected and compared .Results The tumorigenic rate were 100% ,20% ,30% and 0% after the SD rats were inoculated with SHZ-88 ,SHZ-88/MIP-1α,SHZ-88/B7-1 and SHZ-88/MIP-1α+B7-1 cells for 10 days .After the transgenic therapy ,the tumor volume of the combination group in 1~4 weeks were significantly smaller than the other groups .The survival time of the combina-tion group was significantly longer than the other groups .The CD4+ ,CD8+ percentage and CD4+ /CD8+ ratio and the levels of IL-2 and IFN-γof peripheral blood in the combination group were significantly higher than the other groups .The differences above were all statistically significant (P<0 .05) .Conclusion Cotransfection of MIP-1αand B7-1 could induce immune response in breast cancer rats and play anti-cancer effects .
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