120例肝功能损伤患者线粒体抗体及其M2亚型检测结果分析

摘要

目的 检测肝功能损伤患者血清中抗线粒体抗体(AMA)、AMA-M2水平,探讨肝功能损伤患者抗线粒体抗体、抗线粒体抗体M2检测对诊断原发性胆汁性肝硬化(PBC)的重要性.方法 应用酶联免疫法检测丙氨酸氨基转移酶(ALT)高于70 U/L的患者血清样本中的AMA-M2,阳性者同时用免疫印迹法进行AMA-M2检测,间接荧光免疫法(IIF)检测AMA.结果 120例血清样本中ELISA法、免疫印迹法AMA-M2阳性6例,间接免疫荧光法AMA均为阳性.结论 临床上应重视对血清AMA、AMA-M2抗体测定,有助于早期PBC的诊断.%Objective To analyzed clinical significance of dectection of antimitochondrial antibody in patients with liver injury,and emphasize the meaning for detecting antimitochondrial antibody in patients with liver injury.Methods Apply Fluorescence immunity technology(IIF)and antigen specificity ELISA indirectly to detect 120 cases that Alanine amino transferase(ALT)was high er than 70,and prove it using immune imprinting method.Results The results of AMA testing were positive in 7 cases;clinical diagnoses separately are Biliary infections(1 case),Pleural bolt compressibility fractures(1 ease),dermatomyositis(1 ease),Multiple organ failure(1 case),primary biliary cirrhosis(1 case),primary hypertension(1 case),drug-induced hepatitis(1 case).Conclusion The dectection of antimitochondrial antibody is worthy of being popularized,since it's beneficial for diagnosing patients with liver injury.