PCNA 、CDK4及 p16INK4a在外阴上皮内非瘤样病变中的表达及意义

摘要

目的：探讨增殖细胞核抗原（PCNA）、细胞周期依赖性激酶（CDK4）及其抑制蛋白p16INK4a在外阴上皮内非瘤样病变（NNEDV）发病中的作用及关系。方法收集 NNEDV 患者外阴病变皮肤（病变组）及病变旁皮肤（病变旁组）标本各52例；同时收集行会阴修补术患者的外阴正常皮肤标本25例（对照组）。免疫组织化学法检测 PCNA 、CDK4及 p16INK4a 在各组中的表达。结果 PCNA 的表达，病变组显著高于病变旁组和对照组（P ＜0．05），病变组3种组织学类型间表达差异无统计学意义（P ＞0．05）；病变旁组略高于对照组（P＞0．05）。 CDK4的表达，病变组和病变旁组均显著高于对照组（P＜0．05），病变组3种组织学类型间表达差异无统计学意义（P＞0．05）；病变组略高于病变旁组（P＞0．05）。 p16INK 4a 的表达，病变组显著低于病变旁组和对照组（P＜0．05），病变组3种组织学类型间表达差异无统计学意义（P＞0．05）；病变旁组略低于对照组（P＞0．05）。 PCNA 和 CDK4的表达在病变组3种组织学类型中均呈正相关（P＜0．05），在病变旁组和对照组中均无相关性（P＞0．05）；PCNA 和 p16INK4a 的表达在病变组3种组织学类型中均呈负相关（P＜0．05），在病变旁组及对照组中均无相关性（P＞0．05）；CDK4和 p16INK4a的表达在各组中均呈不相关（P＞0．05）。结论 PCNA 、CDK4及 P16INK4a的表达与 NNEDV 细胞增殖和细胞增殖周期的调控相关，很可能成为治疗 NNEDV 的分子靶点。%Objective To study the expression and significance of proliferation cell nuclear antigen (PCNA) ,cyclin dependent ki‐nase 4(CDK4) and p16INK4a in nonneoplastic epithelial disorders of vulvar(NNEDV) and explore the correlation of them .Methods Fifty‐two vulvar lesion samples(lesion group) and 52 vulvar skin surrounding the lesions samples(lesion‐adjacent group) were col‐lected from NNEDV patients ,and lesion group include 27 samples with squamous hyperplasia of vulvar(VSH) ,15 samples with li‐chen sclerosus of vulvar(VLS) and 10 samples containing both lesions(mixed‐lesion) .25 vulvar skin samples were collected from patients receiving perineal repair operation as control group .The expression of PCNA ,CDK4 and p16INK4a was detected by immuno‐histochemistry assay .Results The expression of PCNA in lesion group was significant higher than those in lesion‐adjacent group and control group (P< 0 .05) ,while there was no significant differences among VSH ,VLS and mixed lesion ,and there was no sig‐nificant difference between lesion adjacent group and control group (P> 0 .05) .The expression of CDK4 in lesion group and lesion‐adjacent group were significant higher than that in control group (P < 0 .05) ,while there weren′t significant differences among VSH ,VLS and mixed‐lesion (P> 0 .05) ,and there was no significant difference between lesions group and lesion‐adjacent group (P> 0 .05) .The expression of p16INK4a in lesion group was significant lower than those in lesion‐adjacent group and control group (P< 0 .05) ,while there was no significant differences among VSH ,VLS and mixed‐lesion (P> 0 .05) ,and there was no significant difference between lesion‐adjacent group and control group (P> 0 .05) .About PCNA and CDK4 ,there was a positive correlation in lesion group (P< 0 .05) and there were no correlations in other groups (P> 0 .05) .About PCNA and p16INK4a ,there was a negative correlation in lesion group (P< 0 .05) and there were no correlations in other groups (P > 0 .05) .About CDK4 and p16INK4a ,there were no correlations in all groups (P> 0 .05) .Conclusion expression of PCNA ,CDK4 and p16INK4a may be related of abnormal in‐creasing of cell proliferation and acceleration the process of cell cycle ,which may be applied to molecular target for treatment .