DR E2基因参与酵母内质网应激调控机制的初步研究

摘要

Objective To study the role of saccharomyces cerevisiae DRE2 in endoplasmic reticulum response induced by tunica‐mycin .Methods The der2 ::URA3 gene deletion cassette was amplified from the wild type genomic DNA by PCR ;DRE2 deficiency heterozygote strain was made by gene recombination .The heterozygote strain resistant ability to tunicamycin and the replicative li‐fespan were analyzed in this study .Results DRE2 deficiency strain was resistant to tunicamycin ,but the replicative lifespan was decreased compared to wild type strain (P< 0 .05) .Conclusion DRE2 may be involved in the yeast endoplasmic reticulum response and replicative lifespan regulation .%目的：初步研究 DRE2基因在抗肿瘤药物衣霉素诱导的内质网应激中的作用机制。方法以质粒pRS306为模板，通过 PCR 扩增含有 URA3开放阅读框的 DRE2基因破坏元件 der2：URA3，然后利用基因重组原理，构建 DRE2基因杂合缺失酵母菌株，并进一步检测其对衣霉素的抗性和复制寿命。结果相对于野生型菌株，DRE2基因杂合缺失酵母菌株对衣霉素的抗性增高，复制寿命缩短（P＜0．05）。结论 DRE2基因可能参与了酵母内质网应激和衰老的调控。