Objective To study and establish the quantitative analysis method for verbascoside ,luteolin and apigenin in Siphono-stegia chinensis by HPLC .Methods HPLC was performed on Kromasil C18 (4 .6 mm × 250 mm ,5 μm) ,the mobile phase consisted of acetonitrile and water(containing 0 .1% phosphoric acid) at a flow rate of 1 .0 mL/min .The column temperature was set at 35℃ ,and the eluate was detected at 340 nm .Results Quantitative analysis of HPLC showed that the linear ranges of verbascoside , luteolin and apigenin were 0 .197 6~1 .482 μg (r=0 .999 8) ,0 .100 8~0 .756 μg (r=0 .999 7) ,0 .043 2~0 .324 μg (r=0 .999 7) respectively .The average recovery of verbascoside ,luteolin and apigenin were 99 .7% (RSD= 2 .0% ) ,99 .5% (RSD = 2 .3% ) , 99 .4% (RSD=2 .2% ) respectively .Conclusion The method is accurate ,feasible and reliable ,therefore it′s available for the quality control of Siphonostegia chinensis .%目的：建立北刘寄奴药材中毛蕊花糖苷、木犀草素和芹菜素的含量测定方法。方法采用Kromasil C18色谱柱（4．6 mm ×250 mm ，5μm）；流动相乙腈与0．1％磷酸（22∶78）；流速1．0 mL/min；柱温35℃；进样量10μL ；检测波长340 nm。结果毛蕊花糖苷、木犀草素和芹菜素分别在0．1976～1．482μg、0．1008～0．756μg和0．0432～0．324μg范围内线性关系良好，回归方程分别为Y＝7．171×105 X＋2．094×103，r＝0．9998、Y＝6．833×105 X＋1．306×103，r＝0．9997、Y ＝7．210×105 X＋9．73×102，r＝0．9997，平均加样回收率分别为99．7％（RSD＝2．0％）、99．5％（RSD＝2．3％）、99．4％（RSD＝2．2％）。结论所建立的方法准确性高，重复性好，可作为北刘寄奴药材的质量控制方法。
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