HPLC-DAD同时测定洋甘菊中木犀草素-7-O-β-D葡萄糖苷和芹菜素-7-O-β-D葡萄糖苷的含量

摘要

目的 建立同时测定洋甘菊中木犀草素-7-O-β-D葡萄糖苷和芹菜素7-O-β-D葡萄糖苷含量测定的HPLC方法.方法 色谱柱:Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm);流动相:甲醇-0.2％磷酸(34∶66);流速:1.0ml/min;柱温25℃;进样量10 μl;检测波长:350 nm.结果 木犀草素-7-O-β-D葡萄糖苷和芹菜素-7-O-β-D葡萄糖苷的线性范围分别为5.11 ～ 409.00、12.56～1005.00μg/ml,相关系数r＞0.999 3,具有良好的线性.精密度、稳定性符合要求,木犀草素-7-O-β-D葡萄糖苷和芹菜素-7-0-β-D葡萄糖苷加样回收率分别为96.44％～103.55％、95.12％～101.52％,RSD均为0.03％.结论 该方法简便、准确、重复性好,适用于洋甘菊中木犀草素-7-O-β-D葡萄糖苷和芹菜素7-O-β-D葡萄糖蔷含量测定.%Objective To establish an HPLC method for simultaneous determination the contents of galuteolin and apigenin 7-O-β-D glucoside in Matricaria recutita simultaneously.Methods The contents of galuteolin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside were determined on an Agilent Zorbax SB-C18 column (4.6 mm × 250 mm,5 μm) with a mobile phase consisting of methanol-0.2％ phosphoric acid (34 ∶ 66) at a flow rate of 1.0 ml/min and detected at a wavelength of 350 nm,column temperature was 30℃.Results The linear range of galuteolin-7-O-β-D glucoside and apigenin-7-0-β-D glucoside was 5.11 ～ 409.00,12.56 ～ 1 005.00 μg/ml,the correlation coefficient r ＞ 0.999 3,has a good linearity.Precision and stability meet the requirements,the recovery was 96.44％ ～ 103.55％ and 95.12％ ～ 101.52％,for galuteolin-7-O-β-D-glucoside,apigenin-7-O-β-D-glucoside,respectively.Conclusion The method is simple,selective and has good repeatability.It can be used for the simultaneous determination of galuteolin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside in Matricaria recutita.