目的：观察加味四物汤对特发性血小板减少性紫癜（ITP）模型小鼠的药效及免疫方面的影响。方法用豚鼠抗小鼠血小板血清（GP‐APS）建立免疫性ITP模型，并用相应药物进行治疗，按用药情况分模型生理盐水组，西药组（泼尼松治疗），中药组（加味四物汤治疗）。观察各组小鼠、骨髓有核细胞计数、血清IL‐6含量变化及胸腺、脾脏指数等的变化情况。结果与模型生理盐水组相比西药组与中药组外周血白细胞（WBC）、血小板（PLT）计数，骨髓有核细胞计数均显著升高（P＜0．01），血清IL‐6含量也显著上升（P＜0．01），胸腺、脾脏指数明显升高（P＜0．05）；西药组和中药组的外周血WBC、PLT，骨髓有核细胞计数，血清IL‐6组间差异无统计意义（P＞0．05）。结论加味四物汤可能通过调节细胞免疫、上调血清IL‐6含量、提高机体免疫功能、改善骨髓造血微环境来实现对ITP模型小鼠的治疗作用。%Objective To observe the effect of Jiawei Siwu Decoction for idiopathic thrombocytopenic purpura (ITP) on the effi‐cacy and immune aspects of the model mice .Methods We used guinea pig anti mouse platelet serum immune IT P model ,then con‐duct the corresponding drug treatment .The mice were observed in peripheral blood ,bone marrow changes of nuclear cell count ,ser‐um IL‐6 content and changes in the spleen index .Results Compared with the model group of physiological saline of peripheral blood WBC ,prednisone group and Chinese medicine group PLT count ,bone marrow nucleated cell counts were significantly elevated (P<0 .01) ,the content of IL‐6 in serum were increased significantly (P<0 .01) ,no difference between the two treatment groups . Thymus and spleen index increased too (P<0 .05) .Conclusion Jiawei Siwu Decoction may be related to the regulation of cellular immunity ,increased serum IL‐6 content ,improve bone marrow hematopoietic microenvironment to achieve therapeutic effects in the ITP mice .
展开▼
