钾离子通道Kv3．4在大鼠颊黏膜癌变中的表达研究

摘要

Objective To investigate the expression of the Kv3 .4 protein and Kv3 .4 mRNA in various stages of oral carcino‐genesis .Methods The expression of Kv3 .4 protein and Kv3 .4 mRNA were detected by immunohistochemistry (SP method) and RT‐PCR technique respectively in the oral carcinogenesis of SD rat which were induced by 4NQO .Results With the aggravation of the epithelial dysplasia ,Kv3 .4 protein and Kv3 .4 mRNA expression increased gradually .They were strongly positive in oral squa‐mous cell carcinoma .Conclusion The expression of Kv3 .4 protein and Kv3 .4 mRNA levels increased consistently with the aggra‐vation of the epithelial dysplasia .%目的：检测Kv3．4蛋白和Kv3．4 mRNA在大鼠颊黏膜癌变过程中的表达水平。方法采用免疫组织化学（SP）法及RT‐PCR技术检测4NQO诱导的58只SD大鼠颊黏膜癌变各阶段组织中Kv3．4蛋白和Kv3．4 mRNA的表达。结果随上皮异常增生程度的增加，Kv3．4蛋白和Kv3．4 mRNA表达逐渐增加，在口腔鳞状细胞癌中呈强阳性表达。结论在大鼠颊黏膜癌变过程中，Kv3．4蛋白和Kv3．4 mRNA呈现出相似的一致性增高的趋势。