S-腺苷蛋氨酸对奈乙硫氰酸酯致大鼠药物型胆汁淤积性肝病肝纤维化的保护作用

摘要

目的：研究 S-腺苷蛋氨酸(SAMe)对奈乙硫氰酸酯(ANIT)诱发大鼠药物型胆汁淤积性肝病肝纤维化的保护作用。方法将18只 SD 大鼠分为3组：A 组(空白对照组)、B 组(造模+NS)、C 组(造模+SAMe),每组各6只,各组大鼠腹腔注射后72 h 处理。采用全自动生化分析仪检测各组肝脏血清学指标,并取肝脏组织行 HE 染色及 MASSON 染色观察肝脏病理组织学变化及胶原含量改变。结果C 组大鼠的血清学指标谷氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(TBiL)、直接胆红素(DBiL)、间接胆红素(IBiL)、谷胺酰转肽酶(γ-GGT)、碱性磷酸酶(ALP)、总胆汁酸(TBA)明显低于 B 组,3组之间比较差异均有统计学意义(P <0.05)；但3组之间总胆固醇(Chol)水平差异无统计学意义(P >0.05)。C 组大鼠的胶原纤维含量及胶原纤维面积比均明显低于 B 组,差异有统计学意义(P <0.05)。结论SAMe 能通过改善药物型胆汁淤积性肝病的肝功能,降低胶原纤维含量及面积,进一步保护肝脏组织。%Objective To study the protective effects of S-adenosyl methionine(SAMe)on liver fibrosis of rats with cholesta-sis induced by alpha-naphthyliso thiocyanate.Methods 18 SD rats were randomly divided into three groups,the group A (blank control group),B [model+ normal saline(NS)]and C (model + SAMe),6 rats in each group.The rats in each group were sacri-ficed at 72 h after intraperitoneal injection.Serum levels of liver indicators in each group were detected by the full automatic bio-chemical analyzer.The liver tissue was taken for conducting HE staining and MASSON staining and observing the pathological changes of livers.Results Compared with the group B,the serum levels of ALT,AST,TBiL,DBiL,IBiL,R-GGT,ALP and TBA in the group C were significantly decreased,the differences among 3 groups were statistically significant(P <0.05);but the total Chol level had no statistical difference among 3 groups(P >0.05).The collagen fibers content (1 75.1 ±127.9)and the collagen fibers area ratio(14.1±6.4)in the group C were significantly lower than those in the group B,the difference was statistically significant (P <0.05).Conclusion SAMe can decrease the content and area of collagen fibers and further protect the liver tissue by improving the liver function in drug induced cholestasis liver disease.