2种检测系统测定血、尿淀粉酶高值标本时稀释液的评价与选择

摘要

目的：对湿生化和干生化检测系统测定血、尿淀粉酶高值标本时的稀释液进行评价与选择，期望筛选出与检测系统和标本类型匹配的稀释液。方法纯水、生理盐水（NS）、7％牛血清清蛋白（BSA）、低值血清、低值尿液作为候选稀释液，稀释对应的标本后在湿生化和干生化系统中测定，以卫计委颁布的最新标准分别对偏差和线性进行评价。结果对高值血清标本，同一稀释液在2个系统间的偏差存在较大差异，在湿生化检测系统中，纯水、7％ BSA、低值血清作稀释液的偏差均小于行业标准规定的总允许误差（15％），但以低值血清最优；在干生化检测系统中，纯水、NS、低值血清作稀释液的偏差均小于行业标准的规定，以NS最优。对高值尿液标本，在湿生化检测系统中，纯水、低值尿液作稀释液的偏差均小于行业标准规定的总允许误差，以低值尿液最优；在干生化检测系统中，仅低值尿液作稀释液的偏差小于行业标准之规定。线性评价显示，NS不适合作高值血清标本在湿生化检测系统和高值尿液标本在干生化系统的稀释液，7％ BSA不适合作高值血清标本在干生化检测系统的稀释液。结论相同稀释液在不同的检测系统中造成的影响不同。高值血清、尿液标本在湿生化检测系统中的最适稀释液分别是低值血清和低值尿液，在干生化检测系统最适稀释液分别是NS和低值尿液。%Objective To evaluate and select the dilution for high blood and urinary amylase among wet chemical & dry chemical de‐tection system ,hope to screen the optimum dilution for different measurement system and specimen type .Methods Pure water (H2 O) ,nor‐mal saline (NS) ,7% bovine serum albumin(7% BSA) ,low amylase specimen of serum and urine selected as candidate dilution .The high amylase specimen was diluted ,then was measured among wet chemical analyzer and dry chemical analyzer ,then the bias and correlation was evaluated according to the professional standard of National health and family planning commission of china .Results For high amylase ser‐um specimen ,the same dilution resulted in different bias between two detecting system .The bias of H2 O ,7% BSA and low amylase serum were less than the TEa (total error allowance) regulated by professional standard in wet chemical analyzer .Of them ,the low amylase serum was the best .In dry chemical analyzer ,the bias of H2 O ,NS and low amylase serum were no less than the professional standard .Of them ,NS was the best .For high amylase urine specimen ,the bias of H2 O and low amylase urine were less than the professional standard in wet chem‐ical analyzer ,and the low amylase urine was the best .In dry chemical analyzer ,only the bias of low amylase urine met the requirement of professional standard .The evaluation of linearity indicated that NS was unfit to the dilution for high amylase serum in wet chemical analyzer and for high amylase urine in dry chemical analyzer ,7% BSA was unsuitable to be the dilution for high amylase serum .Conclusion The effect of same dilution on different detecting system is different .The most suitable dilution is low amylase serum and urine for high amylase serum and urine specimen respectively in wet chemical analyzer ,and the suitable dilution is NS and low amylase urine respectively in dry chemical analyzer .