Objective To observe the effects of naringin (Nar) on hemodynamics in myocardial ischemia-reperfusion(MI/R) injury rats for investigating its protective role on MI/R rats.Methods The ischemia-reperfusion(I/R) model was prepared by ligating the left anterior descending coronary artery,meanwhile different doses of Nar(10,20,40 mg/kg) were given.Then the electrocardiogram (ECG) was recorded after 7 d.The left ventricular end diastolic pressure (LVEDP),left ventricular systolic pressure (LVSP) and maximum ascending rate of left ventricular pressure/maximal descending rate of left ventricular pressure (±dp/dtmax) were measured.The activities of srum creatine kinase (CK) and lactate dehydrogenase (LDH) were measured by using the chemical colorimetry method.Results Compared with the MI/R group,Nar 20,40 mg/kg could decrease LVEDP,increased ±dp/ dtmax and LVSP,and decreased the activities of serum CK and LDH (P＜0.05),showing a dose-dependent manner.Conclusion Nar has the protective effect against MI/R injury rat and alleviates the myocardial damage extent,which may be related with improving the hemodynamics.%目的 观察柚皮苷对心肌缺血/再灌注损伤(MI/R)大鼠血流动力学的影响,探讨其对MI/R大鼠的保护作用.方法 采用结扎大鼠冠状动脉左前降支制备MI/R模型,同时给予不同剂量的柚皮苷(10、20、40mg/kg),7d后测定心电图,记录缺血前、缺血30 min及再灌注不同时间点的左室舒张末期内压(LVEDP)、左室收缩内压(LVSP)、左室内压最大上升/下降速率(±dp/dtmax);化学比色法测定动脉血清肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性.结果 与MI/R组相比,柚皮苷20、40mg/kg可使LVEDP降低,±dp/dtmax和LVSP升高,血清中CK和LDH活性降低(P＜0.05),呈现剂量依赖性.结论 柚皮苷对大鼠MI/R具有保护作用,减轻心肌损伤程度.,可能与改善血流动力学有关.
展开▼
