By the technique of protoplast fusion，interspecific fusantsbetween Pseudomanos SY-l and Norcardia RB-1 had been obtained．The superior conditions of protoplast formation，protoplast fusion and wastewater treatment using the fusants were studied．It is better to prepare the protoplast by using lysozyme 1．5mg／ml，10min．The protoplast formation rates of Pseudomamos and Norcardia were 33．3％ and 44．4％ respectively．The fusion was carried out in NSM containing 30％ PEG（MW6000） and the fusion frequency was about 0．3％．At last，the stable fusants were sued to treat the bleaching wastewater．It is obtained that the removal rates of CODCr and TCL are respectively 72．05％ and 190％ higher than that of the mixed parent strains．%采用原生质体融合技术,将两株具有含氯有机化合物降解特性的菌-SY-1和RB-1融合构建成一株高效降解含氯有机化合物的工程菌。实验得出:当溶菌酶浓度为1.5mg／ml，酶解60min时,假单胞菌和诺卡氏菌的原生质体形成率分别为33.3%和44.4%;在30%PEG6000助融下10min二者的融合率为0.3%。应用于造纸漂白废水,融合菌去除CODCr和TCL的能力比混合菌分别提高了72.05%和190%
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