高效液相色谱法测定碳酸钙D3咀嚼片中维生素D3的含量

摘要

Objective: To establish a method for the determination of vitamin D3 in Calcium Carbonate and Vitamin D3 Chewable Tablets by HPLC. Methods:The HPLC system consisted of Diamonsil C18 col-umn(250 mm × 4. 6 mm, 5 μm), acetonitrile-water(23∶77);flow rate at 1. 0ml·min-1 and detected with UV at 264 nm. Results:The method had good linear relationship within the rage 4. 8~38. 4 μg of vitamin D3(r=1. 0). The result (n=6)of the recovery was 97. 82%(RSD=1. 15%. Conclusion:The method is simple, fast, rapid, accurate and suitable for the determination of preparation.%目的：：建立高效液相色谱法测定碳酸钙D3咀嚼片中维生素D3含量的方法。方法：采用高效液相色谱法。以Diamonsil C18(250 mm ×4.6mm,5μm)为色谱柱；流动相为甲醇；流速：1.0 ml·min-1；检测波长：264 nm。结果：维生素D3在4.8~38.4μg范围内。进样量与峰面积线性关系良好(r=1.0)。平均回收率为：97.82%(RSD=1.15%)。结论：方法简便、快速准确、灵敏度高,重现性好。可作为碳酸钙D3咀嚼片的质量控制方法。