川芎嗪对糖尿病肾病大鼠血液流变学、醛糖还原酶及肾功能的影响

摘要

目的 探讨川芎嗪对糖尿病肾病(DN)大鼠血液流变学、醛糖还原酶(AR)及肾功能的影响.方法 腹腔注射链脲佐菌素(55 mg/kg)建立DN大鼠模型,大鼠随机分为5组,模型组,厄贝沙坦组[50 mg/(kg·d)],川芎嗪低、中、高剂量[200、100、50 mg/(kg·d)]组,另设正常对照组.所有大鼠每天灌胃,连续8周.实验结束后,观察血液流变学、血糖、红细胞和肾组织中醛糖还原酶、24h尿蛋白、血尿素氮、肌酐、肌酐清除率、肾功能.结果 与模型组相比,各治疗组中血液流变学、血糖、肾功能均得到有效改善,醛糖还原酶活性显著降低(P＜0.05).HE、PAS染色显示,肾脏病理改变显著减轻.结论 川芎嗪可通过改善血液流变学、降低血糖、抑制醛糖还原酶活性来保护DN大鼠肾脏.%AIM To explore the effects of ligustrazine on blood rheology,aldose reductase (AR) and renal function in diabetic nephropathy (DN) rats.METHODS The DN rat model was established by intraperitoneal injection of streptozotoein (55 mg/kg),rats were randomly divided into five groups,model group,irbesartan [50 mg/(kg · d)] group,high-,middle-and low-dose of ligustrazine [200,100,50 mg/(kg · d)] groups,together with normal control group.All the rats received daily garage for eight successive weeks.At the end of experiment,blood rheology,blood glucose,aldose reductase in erythrocyte and kidney tissue,24 h urinary protein,blood urea nitrogen,creatinine,creatinine clearance and renal function were observed.RESULTS Compared with the model group,blood rheology,blood glucose and renal function in various treatment groups were effectively improved,and aldose reductase activity was significantly decreased (P ＜ 0.05).HE staining and PAS staining showed that the pathological changes in kidney were significantly alleviated.CONCLUSION Ligustrazine can protect kidney of DN rats by ameliorating blood rheology,decreasing blood glucose and inhibiting aldose reductase activity.