HPLC法测定远志及其3种炮制品中3种寡糖酯

摘要

AIM To determine the contents of arillanin A,tenuifoliside A and tenuifoliside C in raw Polygalae Radix (root barks),Polygalae Radix duramen,Glycyrrhizae Radix et Rhizoma-processed Polygalae Radix,waterboiling Polygalae Radix and honey-processed Polygalae Radix.METHODS The analyses of 50％ methanol extracts from samples were performed on a 30 ℃ thermostatic Kromasil C18 column (250 mm ×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1％ formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 330 nm.RESULTS The contents of three oligosaccharide esters were the highest in raw Polygalae Radix,followed by those in honey-processed Polygalae Radix,and those in water-boiling Polygalae Radix were the lowest.These constituents also existed in Polygalae Radix duramen,but their contents were lower than those in root barks.CONCLUSION The ester bonds of oligosaccharide esters in Polygalae Radix may be hydrolyzed during processing,followed by the generation of small molecular organic acids.The medication of whole Polygalae Radix (root barks and duramen) can be taken into consideration in clinical practice to reduce toxicity and enhance efficacy.%目的 通过HPLC法测定生远志(根皮)、远志木心、甘草制远志、清水煮远志、蜜炙远志中黄花远志素、远志蔗糖酯A、远志蔗糖酯C的含有量.方法 样品50％甲醇提取液的分析采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm);流动相乙腈-0.1％甲酸,梯度洗脱;检测波长330 nm;柱温30C;体积流量1.0 mL/min.结果 生远志中3种寡糖酯含有量最高,蜜炙远志次之,清水煮远志最低.远志木心中也存在这些成分,但其含有量均低于根皮中.结论 远志寡糖酯在炮制过程中可能发生酯键水解,生成小分子有机酸.临床上,可考虑全远志(根皮与木心)用药以减毒增效.