银柴胡化学成分及其抗炎活性

摘要

目的 研究银柴胡Stellaria dichotoma L.var.lanceolata Bge的化学成分及其抗炎活性.方法 银柴胡95％乙醇提取物采用硅胶、MCI、反相C18制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构.采用脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7炎症反应模型评价其抗炎活性.结果 从中分离得到10个化合物,分别鉴定为3-羟基-β-卡巴林(1)、taraxacine A(2)、1,2,3,4-四氢-1,3,4-三羟基-β-卡巴林(3)、1-乙酰基-β-卡巴林(4)、arenarine A(5)、arenarine B(6)、邻苯二甲酸二异丁酯(7)、邻苯二甲酸二丁酯(8)、亚麻酸甲酯(9)、苜蓿素(10).化合物1～6对炎症因子有较强的抑制作用,IC504.79 ～ 9.34 μg/mL.结论 所有化合物均为首次从该植物中分离得到,首次发现化合物1～6有较强的抗炎活性.%AIM To study the chemical constituents from the Stellaria dichotoma L.var.lanceolata Bge and their anti-inflammatory activities.METHODS The 95％ ethnol extract from S.dichotoma was isolated and purified by silica,MCI,C18 prep-HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.Their anti-inflammatory activities were evaluated by LPS induced RAW264.7 inflammatory cell model.RESULTS Ten compounds were isolated and identified as 3-hydroxy-β-carboline (1),taraxacine A (2),1,2,3,4-tetrahydro-1,3,4-trioxo-β-carbo1ine (3),1-acetyl-β-carboline (4),arenarine A (5),arenarine B (6),diisobutyl-phthalate (7),dibutyl-phthalate (8),(z,z,z)-9,12,15-octadecatrienoic acid,methyl ester (9),tricin (10).Compounds 1-6 had stronger anti-inflammatory activities with the IC50 values of 4.79-9.34 μg/mL.CONCLUSION All the compounds are isolated from this plant for the first time.Their stronger anti-inflammatory activities are discovered for the first time.