抑制烟草花叶病毒活性植物的筛选

摘要

In this paper, screening of plants inhibiting Tobacco Mosaic Virus (TMV) activity was achieved under the disease measuring standard with half leaf lesion experiment. Botanical extracts were obtained from 24 kinds of plants, including Artemisia Argyi, Radix Isatis, Bupleurum, etc, and their inhibiting effect on TMV activity were tested by passive activation of TMV in vitro and inhibiting TMV primary infection. Pot experiments were used to investigate the protection and therapeutic effect of virus. The results indicated that the abstracts from ginkgo, gardenia, phytolacca and red peony root had great inhibiting effect on TMV, which is promising for further development and utilization.%本研究采用半叶枯斑法和烟草病害分级标准，对抗TMV的活性植物进行筛选，系统测定了艾蒿、板蓝根、柴胡等24种植物的粗提取物对烟草花叶病毒（TMV）的抑制效果，包括体外钝化TMV作用、抑制TMV初侵染；并采用盆栽法，研究植物粗提取物对烟草花叶病的保护和治疗作用等。试验表明，银杏、栀子、商陆、赤芍等植物粗提取物的综合防治效果较好，有进一步开发和利用的前景。