人参皂苷Rg3对子宫内膜异位症组织中ID-1和NRP-1基因表达的影响

摘要

目的 探讨人参皂苷Rg3对子宫内膜异位症(Ems) 组织中ID-1和NRP-1基因表达的影响.方法 采用SuperArray功能分类基因芯片检测的方法筛选Ems血管生成相关基因,并用反转录-聚合酶链反应(RT-PCR)方法进行验证.结果 促血管生成的ID-1和NRP-1基因在异位子宫内膜细胞中表达明显高于在位子宫内膜细胞及正常子宫内膜细胞(P＜0.05);经人参皂苷Rg3作用后ID-1和NRP1基因在异位子宫内膜细胞中表达明显低于在位子宫内膜细胞及正常子宫内膜细胞(P＜0.05).结论 人参皂苷Rg3有抑制子宫内膜异位细胞中ID-1和NRP1基因表达的作用,ID-1和NRP1基因可能在Ems血管生成中发挥重要作用.%Objective To study the influence of' ginsenoside Rg3 on angiogenetic ID-1 and NRP-1 gene exrnpression in endometriotic tissues (EMs). Methods SuperArray gene chip functional classification was used to screen rnEMs angiogenesis related genes, and RT-PCR was used for verification. Results The expression of angiogenesis ID-1 rnand NRP1 gene of ectopic endometrial cells is obviously higher than normal endometrial cells (P＜0.05), but that of ecrntopic endometrial cells is obviously lower than normal endometrial cells after ginsenoside Rg3 treatment (P＜0.05).rnConclusion Ginsenoside Rg3 may inhibit the expression of ID-1 and NRP1 gene in endometriosis and it might play rnan important role on angiogenesis in endometriosis.