腭肺及鼻咽上皮克隆蛋白和Toll样受体-4在鼻息肉组织中的表达及相关性分析

摘要

Objective To investigate the difference in and correlation of PLUNC and toll-like receptor 4 (TLR-4) expression in nasal polyps tissue and normal nasal mucosa by immunoblotting techniques, and to determine the roles of these two markers in the pathogenesis of nasal polyps. Methods Forty-five specimens of nasal polyps and 15 of normal nasal mucosa were biopsied for Western blotting for semiquantitative assessment of PLUNC and TLR-4 ex-pression. The correlation between the two markers was also evaluated. Data were analyzed by using the SPSS 17.0 software. Results Compared with controls, the expression of TLR-4 was significantly increased and PLUNC reduced in nasal polyps (both P<0.05). There was negative correlation between PLUNC and TLR-4 in nasal polyps (r=-0.384, P<0.05). Conclusion Infective factors may play an important role in the pathogenesis of nasal polyps. The existence of infective factors (esp. Gram-negative bacteria) may increase TLR-4 expression in the nasal mucosa, which suppress PLUNC secretion leading to the development of nasal polyps.%目的：运用蛋白印迹法明确腭、肺及鼻咽上皮克隆（PLUNC）蛋白和TLR-4在鼻息肉和正常鼻黏膜组织之间的表达差异和相关性，进一步探讨二者在鼻息肉病因学及疾病进展中的作用。方法采用蛋白印迹法分别半定量检测45例鼻息肉和15名正常鼻黏膜组织中PLUNC蛋白和TLR-4的表达情况，并分析二者在鼻息肉组织中表达的相关性。结果①鼻息肉组与对照组鼻黏膜组织之间PLUNC蛋白和TLR-4表达差异均有统计学意义（P＜0．05），鼻息肉组织中TLR-4高表达，PLUNC蛋白低表达；②鼻息肉组PLUNC蛋白和TLR-4表达呈负相关性（r＝-0．384，P＜0．05）。结论感染性因素在鼻息肉的发病过程中起着重要的作用，感染性因素的存在（特别是革兰阴性菌）使得鼻黏膜TLR-4表达量增高，鼻黏膜长期处于慢性炎症反应使得PLUNC表达分泌下降，可能促进了鼻息肉形成。