藏羊血液中血红素的制备

摘要

To establish a new extract method of Tibetan sheep heme.The crude extracts of Tibetan sheep blood as the raw material,acetone and acid acetone as solvent,was purified.Infrared spectroscopy was applied for qualitative analysis of heme,and ultraviolet spectrophotometry was utilized for quantitative determination of heme.By single-factor test to determine that the dosage of acetone is blood volume of 1 times and centrifugation time is 15min,which is the optimal preparation conditions of Tibetan sheep heme.The method of preparation of heme makes the low-cost,simple operation,short production cycle,purity and yield can reach respectively 98.60% and（87.91±9.04）%.%建立一种新型藏羊血红素的提取方法。以藏羊血为原料,丙酮和酸性丙酮为提取溶剂,所得粗提物进行纯化后,采用红外光谱法对血红素进行定性分析,并用紫外分光光度法进行定量测定。通过进行单因素试验,可确定丙酮添加量为血液体积1倍、离心时间为15min是制备藏羊血红素的最优条件,该法制备血红素成本低,操作过程简便,生产周期短,纯度可达98.60%,且收率达到（87.91±9.04）%。