二倍体马铃薯体细胞电融合参数的优化

摘要

以有性杂交不亲和的抗虫、抗晚疫病的野生二倍体马铃薯Solanumpinnatisecta（2n=2x=24）和抗晚疫病的二倍体材料DY4—5—10（2n=2x=24）的无菌苗叶片为原生质体来源，进行了电融合的研究，以优化马铃薯高效融合的参数。研究结果表明：其最佳的电融合参数为预融合电压1．0×105V／m，时间60s，脉冲强度1．8×106V／m，脉冲宽幅20μs，脉冲次数1次。其有效融合率为28．89％，破损率为13．33％。%This study attempted to optimize the fusion parameters using mesophyll protoplasts isolated from diploid wild species So/anum pinnatisecta (2n = 2x = 24), which is sexual incompatible with cultivated tetraploid potatoes and resistant to insect and late blight, and a diploid clone DY4-5-10 (2n = 2x = 24), which is resistant to late blight. The results showed that the optimal parameters for electrofusion were: alternating current (AC) 1.0 × 105 V/m, AC duration 60 s, direct current (DC) 1.8 × 106 V/m, pulse width 20μS, and number of pulses 1. Under these conditions, the effective fusion rate reached 28.89%, and the breakage rate was 13.33%.