利用HPLC法测定不同马铃薯品种茎叶中的α-茄碱含量

摘要

Stem and leaf of potato have high feeding values, but high content ofα-solanine in potato stem and leaf, which leads to poor palatability and difficulty in silage storage, is the main factor affecting utilization for potato stem and leaf. Understanding the content changes inα-solanine of stem and leaf could provide technical support for their safe use. After harvesting, stem and leaf of potato were dried and crushed, the mixed samples were extracted with ethanol acetic forα-solanine, and then high performance liquid chromatography (HPLC method) was used to determine its changes in different potato varieties. The results showed that theα-solanine contents of stem and leaf in 'Favorita', 'Kexin 2', 'Longshu 3', 'Longshu 6', and 'Longshu 7' were 0.138, 0.171, 0.159, 0.185 and 0.199 mg/g, respectively. Theα-solanine content of stems and leaves has genotypic difference.%马铃薯茎叶具有较高的饲用价值，但马铃薯茎叶中α-茄碱（龙葵素）含量高，适口性差，不易青贮，是影响马铃薯茎叶利用的主要因素。研究马铃薯茎叶中龙葵素的含量变化可为其利用提供安全技术保障。将马铃薯茎叶收获后风干粉碎，用乙醇-乙酸法提取，高效液相色谱法(HPLC法)测定混合样品中的α-茄碱含量，研究不同马铃薯品种茎叶中α-茄碱含量的差异。结果表明：‘费乌瑞它’、‘克新2号’、‘陇薯3号’、‘陇薯6号’、‘陇薯7号’5个马铃薯品种茎叶样品中α-茄碱的含量分别为0.138，0.171，0.159，0.185和0.199 mg/g。茎叶中α-茄碱的含量存在基因型差异。