Genetic polymorphism of human glutathione S-transferase A1 gene in mainland Chinese and its association with phenotype

摘要

AIM Human glutathione S-transferase A1 (GSTA1) is an important phase Ⅱ metabolizing enzyme involved in the metabolism of many therapeutic drugs and is responsible for the metabolic detoxification of numerous promutagens and procarcinogens. The genetic polymorphism of GSTA1 has important implications for drug efficacy and cancer susceptibility. In this study, we determined the distribution of GSTA1 genetic polymorphism in Mainland Chinese. And we also investigated whether there exists the potential phenotype alterations caused by the genetic polymorphism in human. METHODS Genomic DNA was ex-tracted from peripheral blood of 140 Chinese people and 16 liver tissues obtained from non-liverish patients who underwent partial hepatectomy. And then the genotypes of human GSTA1 gene were analyzed by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP).