Aim To explore the effect of bone morpho-genetic protein-7 ( BMP-7 )on expressions of nestin and glial fiberillary acidic protein ( GFAP )in brain tissue of rat after cerebral ischemia reperfusion injury. Methods Ten rats from 40 healthy adult male Sprague-Dawley rats received sham-operation. The other rats were subjected to middle cerebral artery occlusion ( MCAO ) for 2 hours. The 20 successfully modeled rats were equally randomized into the treatment group and control group. Rats in the treatment group were inter-vened with BMP-7 ( 0. 1 mg . Kg-1 ) 250 jjlI via caudal vein injection, while rats in control group and sham-operation group were injected with equal volume of sterile water for injection. Neurological deficits were e-valuated by Bederson's method at 24 h after ischemia-reperfusion. The infarction volume of brain was investigated by 2,3 ,5-triphenyl tetrazolium chloride coloring. The neuronal apoptosis was assessed by TUNEL staining and the expression of nestin and GFAP of the three groups were analyzed by immunohistochemistrymethod. Results The Bederson's score( t =4. 66, P < 0. 01 ) and focus infarction( t = 6. 98 ,P < 0. 01 )were lower in BMP-7 treatment group than those in control group. Besides, the number of TUNEL positive cells in treatment group was lower than those in control group ( P <0. 01 ). Compared with the control group, the expression of nestin and GFAP enhanced in BMP-7 treat-ment group ( P <0. 01 ). Conclusion BMP-7 can up-regulate the expression of nestin and GFAP, and promote neural regeneration to protect animals against ischemia injury.%目的 观察骨形态发生蛋白-7(BMP-7)对大鼠局灶性脑缺血/再灌注损伤后巢蛋白(nestin)和胶质纤维酸性蛋白(GFAP)表达的影响.方法 成年♂SD大鼠40只,随机分为假手术组、对照组、BMP-7治疗组,每组10只.应用线栓法制备大脑中动脉阻塞2 h模型,治疗组尾静脉注射BMP-7(0.1 mg·kg-1)250 μl,对照组和假手术组尾静脉注射等量的无菌注射用水.再灌注24h后行神经功能评分,2,3,5-三苯四氮唑(TTC)染色观察脑梗死体积,TUNEL法检测细胞凋亡,免疫组织化学方法测定nestin和GFAP表达.结果 与对照组相比,BMP-7组大鼠神经功能评分(t=4.66,P<0.01)和脑梗死体积百分比降低(t=6.98,P<0.01),TUNEL阳性细胞数降低(P<0.01).缺血侧脑组织nestin和GFAP表达增加(P<0.01).结论 BMP-7可上调大鼠脑缺血/再灌注损伤后nestin和GFAP表达,促进神经再生,起神经保护作用.
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