HBx蛋白对α干扰素诱导的MxA蛋白表达的影响

摘要

Aim To explore the effects of Hepatitis B virus X( HBx ) protein on the antiviral proteins induced by Interferon-a and its possible mechanisms. Methods HepG2 cells were transfected with FL1-145HBx plasmid, and the mRNA levels of MxA. STATI were assessed by RT-PCR after treated with IFN-α. Meanwhile. the expression of HBx,p-ERK, p-STATI and tSTATI proteins were detected by Western blot. Results The expression levels of total STAT1, phosphorylated STATI and STATI mRNA were down-significantly in transfected cells( P ＜ 0. 05 ). However. these proteins were restored to the expression levels of control groups when the transfected cells were pretreated by ERK inhibitor PD98059. Conclusion HBx protein probably influences the expression of IFN-α JAK-STAT signal pathway molecules and antiviral protein MxA.Moreover, the activation of ERK signal transduction pathway may be involved in the procedure.%目的 探讨乙型肝炎病毒(HBV)HBx蛋白(Hepatitis B virus X protein)对α干扰素(IFN-α)诱导的抗病毒蛋白的影响及相关机制.方法 以表达HBx蛋白的重组质粒FL1-145HBx转染人肝胚瘤细胞株HepG2细胞,经IFN-α处理后,RT-PCR法分析细胞内抗病毒蛋白MxA和JAK-STAT信号转导途径分子STAT1 mRNA表达水平,同时运用免疫印迹检测细胞内HBx、p-ERK、p-STAT1和t-STAT1等蛋白的表达.结果 转染细胞内MxA、STAT1的mRNA和p-STAT1、t-STAT1的蛋白表达水平明显减少(P＜0.05);而ERK抑制剂PD98059预处理后,转染细胞内MxA、STAT1 mRNA水平能够恢复至转染前的表达水平.结论 HBx蛋白很可能通过影响IFN-α JAK-STAT信号转导途径分子而抑制抗病毒蛋白MxA的表达;ERK信号转导途径的活化可能参与这一抑制过程.