Aim To explore the intervention mechanisms of baixiangdan capsule by observing the changes of the expression level of 5-HTR2C and the concentration of IP3 in neonate rat hippocampal neurons culturedin vitro. Methods Premenstrual syndrome ( PMS ) model rats with liver-qi invasion were induced by multiple emotional stimulations and baixiangdan capsule was administered after PMS induction. The medicatedserum was used to neonate rat hippocampal neurons . The expression level of 5-HTR2C in the neurons was e- valuated by Western blot and the concentration of IP 3 was measured with the Elisa method. Results Compared with control group , both the protein expression of 5-HTR2C and the concentration of IP 3 of PMS liver-qi invation group were significantly higher in neurons (P < 0. 01, P < 0. 01 ). After treated with baixiangdan medicated serum, the expression of 5-HTR2C and the concentration of IP3 in hippocampal neurons were significantly improved(P<0. 01,P<0. 01). ConclusionsPMS model rats with liver-qi invasion serum can up-regulate the expression of 5-HTR2C in hippocampal neurons and increase the concentration of the second messenger IP3. Baixiangdan capsule might partially play its role in ameliorating PMS by regulating the expression of 5-HTR2C and changing the concentration of IP3.%目的 观察白香丹胶囊含药血清对原代海马神经元中5-羟色胺2C受体(5-HTR2C)蛋白表达水平及下游信号通路中IP3含量的影响,探讨其可能的中枢作用机制.方法 采用情志刺激为主多因素造模法制备经前期综合征(PMS)肝气逆证大鼠模型,给予白香丹干预,制备大鼠含药血清,结合中药血清药理学,对体外培养的新生大鼠原代海马神经元进行不同血清干预,运用Western blot方法检测海马神经元中5-HTR2C蛋白表达水平,运用ELISA法检测各组海马神经细胞内IP3的含量.结果 与正常血清组相比,PMS肝气逆模型血清组海马神经元的5-HTR2C蛋白表达水平和下游信号通路中IP3含量均明显升高(P<0.01,P<0.01),白香丹含药血清能够明显降低5-HTR2C蛋白表达水平和IP3的含量(P<0.01,P<0.01).结论 PMS肝气逆证模型大鼠血清使海马神经元中5-HTR2C蛋白表达异常升高,同时,可引起第二信使IP3含量升高;白香丹胶囊可能通过降低5-HTR2C蛋白表达水平,改变其下游信号通路中IP3的含量而发挥疗效.
展开▼
