大黄素对P2X2/3受体介导的三叉神经痛的作用研究

摘要

目的 探究大黄素(emodin)对P2X2/3受体介导的三叉神经痛的作用影响.方法 慢性压迫性损伤大鼠眶下神经(CCI-ION)建立大鼠三叉神经痛动物模型,应用von Frey细丝法测试机械痛敏阈值,观察大黄素对三叉神经痛(TN)大鼠机械痛敏阈值的影响.结合原位杂交、PT-PCR、免疫组织化学、免疫荧光、蛋白印迹等方法观察大鼠三叉神经节(TG)中P2X2/3受体的表达变化.结果术后14 d,Ⅲ组(TN模型组)和Ⅰ组(生理盐水对照组)、Ⅱ组(假手术组)、Ⅳ组(TN模型+大黄素干预组)相比较,大鼠手术侧眶下神经面部感觉区域机械痛敏阈值明显降低(P0.05);Ⅳ组TG中P2X2/3受体的表达较Ⅲ组低(P 0. 05 ); the expression of KX2/3 receptor of TG in IV group was lower than that in M group( P < 0. 01 ). Conclusion P2X2/3 receptor may play a key role in the pain transmitting of trigeminal neuralgia, and emodin has inhibitory effect on trigeminal neuralgia.