匹诺塞林对急性局灶性脑缺血大鼠脑线粒体功能的影响

摘要

Aim To investigate the effects of pinocem-brin on brain mitochondria and energy metabolism in rats subjected to middle cerebral artery occlusion ( MCAO ). Methods The MCA in rats was permanently occluded. Pinocembrin ( 1, 3, 10 mg ? Kg'1 ) or Nimodipine ( 3 mg ? Kg"1 ) were injected at 10 min and 12 h after MCAO surgery. 24 h later, activity of LDH, content of ATP, ADP, AMP and CrP in brain tissue were determined. Mitochondria swelling degree, content of calcium, superoxide anion and Mn-SOD, activity of Na + , K + -ATPase and Ca + -ATPase were also detected to evaluate mitochondria function. Results Pinocembrin (3, 10 mg ? Kg'1 ) injection reducedthe activity of LDH, improved the energy load in brain significantly ( P < 0. 05 ). Mitochondria swelling degree , content of calcium and superoxide anion in mitochondria also decreased ( P < 0. 05 ) in pinocembrin-treated groups. The activity of Mn-SOD, Na+ ,K +-ATPase and Ca2 + -ATPase in brain mitochondria were increased remarkably in pinocembrin (10 mg ? Kg'1 ) injected group( P < 0. 05 ). Conclusion Pinocembrin can improve the energy metabolism and anti-ischemia ability in MCAO rats, which may be due to its protective effects on brain mitochondria.%目的 考察匹诺塞林对大鼠急性局灶性脑缺血组织能量代谢及线粒体功能的影响.方法 制备永久性大鼠大脑中动脉栓塞(pMCAO)模型,大鼠随机分为假手术组、模型组、匹诺塞林给药组(1、3、10 mg·kg-1)、尼莫地平给药组(3 mg·kg-1).给药组于手术后10 min、12 h分别给予静脉注射,假手术组与模型组给予生理盐水.于手术24 h应用生化方法检测脑组织中LDH的活性,HPLC法检测大鼠脑组织ATP、ADP、AMP、磷酸肌酸含量.通过检测线粒体超氧化物歧化酶(Mn-SOD)、线粒体Ca2+-ATP酶活性,超氧阴离子含量、线粒体膜肿胀度、线粒体钙离子含量综合评价线粒体功能.结果 匹诺塞林(3、10 mg·kg-1)给药组大鼠缺血脑组织中乳酸脱氢酶活性降低,能量指数比模型组分别提高了34.6%和45.8%(P<0.05);匹诺塞林(10 mg·kg-1)组线粒体SOD 活性提高了24.4 %、Na+,K+-ATP 酶、Ca2+-ATP 酶活性分别升高了24.9 %及34.0 %,线粒体钙离子浓度降低了22.0 %,与模型组相比差异均具有显著性(P<0.05).匹诺塞林(3、10 mg·kg-1)组线粒体超氧阴离子水平分别降低了20.2 %及30.6%、线粒体肿胀程度分别降低了26.1 %及46.0 %,与模型组相比差异均具有显著性(P<0.05).结论 匹诺塞林可减轻大鼠脑缺血后能量损伤,提高脑组织抗缺血缺氧能力,其对线粒体功能的保护作用可能是改善缺血脑组织能量代谢的重要机制.