目的：研究齐墩果酸衍生物Bio对HepG2细胞胰岛素抵抗的改善作用，并探讨其作用机制。方法通过高浓度胰岛素诱导HepG2细胞，建立胰岛素抵抗模型。采用葡萄糖氧化酶法，检测不同浓度化合物对HepG2细胞胰岛素抵抗模型的葡萄糖消耗的影响。 RT-PCR测定化合物对 PPARγmRNA转录水平的影响。 Western blot检测化合物对PPARγ蛋白表达的影响。结果 HepG2细胞经1．72×10-5 mol · L-1的胰岛素诱导后，葡萄糖消耗量明显降低(P<0．05)，胰岛素抵抗模型建立成功。10-5、10-6、10-7 mol · L-1的 Bio均可增加HepG2胰岛素抵抗细胞的葡萄糖消耗( P<0．05)，增加率分别为135%、62%、39%。 RT-PCR检测显示Bio可提高胰岛素抵抗细胞PPARγmRNA的表达。 Western blot结果显示Bio可上调胰岛素抵抗细胞中PPARγ蛋白的表达。结论 Bio对HepG2细胞的胰岛素抵抗具有改善作用，其作用机制与上调PPARγ的表达相关。%Aim To detect the effect of Bio on impro-ving insulin resistance and explore its molecular mech-anism. Methods The HepG2 liver cells were derivat-ed by high concentration insulin to establish the insulin resistance cell model, and the cells were intervened by Bio. The glucose consumption was measured by glu-cose oxidase and peroxidase ( GOD-POD) assay. The expression of PPARγmRNA was detected by RT-PCR. The expression of PPARγ protein was detected by Western blot method. Results The glucose consump-tion was significantly decreased in the insulin resist-ance cells after incubated with 1 . 72 × 10 -5 mol · L-1 insulin ( P<0. 05 ) . 10 -5 ,10 -6 ,10 -7 mol · L-1 Bio increased the glucose consumption 135%,62%,39%separately in the insulin resistance cells. RT-PCR a-nalysis of PPARγ showed that Bio raised the PPARγmRNA. Western blot analysis displayed that the pro-tein of PPARγ with Bio was increased. Conclusion Bio can improve the insulin resistance of the HepG2 cells, and the molecular mechanism may be relevant with raising PPARγ expression.
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