两色金鸡菊醇提物对自发性高血压大鼠尿液的代谢组学研究

摘要

Aim To investigate the effects of the alco-hol extract from Coreopsis tinctoria Nutt. on urinary metabolomics of spontaneous hypertension rats ( SHR) . Methods SHR were fed with normal diet for 1 week and then, they were randomly divided into six groups:untreated control, the high, middle, low dos-age group of the alcohol extract(3.2 g·kg-1 ,1.6 g· kg-1,0.8 g·kg-1) , captopril group(4 mg·kg-1) and Duzhong tablet group(187.5 mg·kg-1). The u-rine of normal rats and SHR hypertension model rats was collected on 1,2,3,4 weeks. The metabolic pro-files were analyzed using 1 H-NMR. PLS-DA methods were used to discriminate the difference and the bio-markers. Results Compared with model group, the blood pressure of all groups was significantly lowered after 4 weeks ( P <0.01 ) . The alcohol extract from Coreopsis tinctoria Nutt. could significantIy reduce blood pressure, and the urinary metabolic profiles trea-ted with Coreopsis tinctoria Nutt. were changed signifi-cantly such as IIL,creatine,β-glucose,etc. Conclusion The alcohol extract from Coreopsis tinctoria Nutt. could significantIy reduce blood pressure and change the urinary metabolomics profiles of SHR rats.%目的：研究两色金鸡菊醇提物对自发性高血压大鼠尿液内源性代谢产物的影响。方法将自发性高血压大鼠( SHR )分为模型组、两色金鸡菊醇提物高、中、低剂量组(3.2、1.6、0.8 g·kg-1)、卡托普利组(4 mg·kg-1)及杜仲平压片组(187.5 mg·kg-1)，连续灌胃给药4周。采用无创血压计观察其对血压的影响；连续4周收集各组大鼠尿液，通过NMR核磁共振氢谱仪对尿液中内源性代谢物进行代谢谱分析，使用SIMCA-P软件进行 PLS-DA分析。结果干预4周后，各给药组大鼠血压均明显下降( P<0.01)；给药组与对照组相比，大鼠尿液代谢物异亮氨酸、肌酸酐、β-葡萄糖、鲨肌醇等有明显变化。结论两色金鸡菊醇提物灌胃对自发性高血压大鼠机体代谢产生了影响，而通过代谢组学发现的差异物质，可以作为标志物进一步阐释两色金鸡菊的药性研究工作并提供实验依据。