白及非多糖组分的止血作用及其机制的初步研究

摘要

目的：明确白及非多糖部分的止血作用，初步了解其止血机制。方法白及95％乙醇提取物上D101大孔树脂，经水洗脱后，用80％乙醇洗脱得到不含多糖的80％乙醇组分（BS－80EE）；以小鼠全身肝素化的出血时间（BT）和凝血时间（CT）为指标考察BS－80EE的止血作用；以二磷酸腺苷（ADP）为诱导剂，采用比浊法观察BS－80EE对大鼠体内血小板聚集率的影响；通过考察BS－80EE对大鼠凝血酶时间（TT）、凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）和纤维蛋白原含量（ FIB），以及血小板膜糖蛋白P－选择素（P－S）、凝血酶－抗凝血酶复合物（TAT）、纤溶酶原激活物抑制剂－1（PAI－1）、D－二聚体（D－D）的影响，分别从血小板和凝血纤溶系统角度了解 BS－80EE 的止血机制。结果 BS－80 EE可剂量依赖性地缩短全身肝素化小鼠的CT和BT （ P＜0．01或P＜0．05）；明显促进ADP诱导的大鼠体内血小板聚集作用（ P＜0．01）；明显缩短大鼠的TT值（ P＜0．01或P＜0．05）；高剂量组可明显增加FIB含量（P＜0．05）；中、高剂量组可明显增加P－S、TAT、PAI－1的含量，减少D－D的生成（P＜0．01）。 BS－80EE虽有缩短大鼠APTT和PT的趋势，但差异均无显著性。结论白及非多糖组分（ BS－80 EE ）具有良好的止血功效，可通过促进血小板聚集和凝血而发挥止血作用。%Aim To understand the hemostatic effect of a non-polysaccharide fraction of Bletilla striata ( BS-80EE) and to clarify its mechanism of action .Methods The non-polysaccharide fraction ( BS-80 EE ) was prepared by passing the 95%ethanol extract of Bletilla striata through a D101 macroporous resin column elu-ted first with water and then with 80%ethanol.Bleed-ing time ( BT ) and clotting time ( CT ) of heparinized mice were employed as indicators for evaluating the he-mostatic effect of BS-80EE.The mechanism of action was investigated through observing the effect of BS-80 EE on platelet aggregation induced by adenosine diphosphate ( ADP) in rats with nephelometry and tes-ting the effect of BS-80EE on the thrombin time(TT), prothrombin time(PT), activated partial thromboplas-tin time(APTT), fibrinogen(FIB), P-selectin(P-S), thrombin-antithrombin complex ( TAT ) , D-dimer ( D-D) and plasminogen activator inhibitor-1 ( PAI-1 ) . Results BS-80 EE significantly shortened the CT and BT( P<0.01 or 0.05 ) of heparin mice in a dose-de-pendent manner; groups of all doses significantly re-duced the rat TT ( P <0.01 or 0.05 ) , and the high-dose group significantly increased the FIB content ( P<0.05); the mid-dose group and high-dose groups of BS-80EE significantly increased the contents of P-S, TAT and PAI-1 , while reduced the D-D production in rats ( P <0.01 ); although dose-dependent reductions of APTT and PT were observed for each treatment-group, no significance was observed .Conclusion BS-80EE possess pronounced hemostatic effect by promo-ting platelet aggregation and coagulation .