OBJECTIVE:To study the hemostatic effect and mechanism of the polysaccharides from Bletilla striata in blood heat and hemorrhage(BBH)model rats. METHODS:Rats were randomly divided into normal control group,model group,Yun-nan baiyao group (0.50 g/kg),and BSP low-dose,medium-dose and high-dose groups (0.34,0.68,1.35 g/kg),with 10 rats in each group. Except for normal control group,those groups were given water decoction of Rhizoma Zingiberis and 5%ethanol to in-duce BBH model. After modeling,those groups were given relevant medicine intragastrically,once a day,for 14 days,except nor-mal control group and model group were given normal saline intragastrically. 1 h after last administration,body weight,food in-take,water intake,the rate of platelet aggregation,the contents of t-PA and PAI-1,pathological change of lung and stomach were all detected in each group. RESULTS:Compared with normal control group,body weight,food intake,maximal rate of platelet ag-gregation and the content of PAI-1 were decreased in model group;while water intake,the content of t-PA and ratio of t-PA/PAI-1 were increased (P<0.05 or P<0.01). Moreover,hemorrhage and inflammation of lung and stomach were found. Compared with model group,the body weight and food intake of Yunnan baiyao group,BSP low-dose,medium-dose and high-dose groups were increased,while water intake were decreased (P<0.05 or P<0.01);the maximal rate of platelet aggregation and the content of PAI-1 were increased in Yunnan baiyao group,BSP medium-dose and high-dose groups,while ratio of t-PA/PAI-1 were decreased (P<0.05 or P<0.01),the pathological changes of lung and stomach relieved to varying degrees. CONCLUSIONS:BSP exerts he-mostatic effect by up-regulating the rate of platelet aggregation,rising fibrinolysis systems,improving the lesion of lung and stom-ach.%目的：研究白及多糖（BSP）对血热出血模型大鼠的止血作用及机制。方法：将大鼠随机分为正常对照组、模型组、云南白药组（0.50 g/kg）和BSP低、中、高剂量组（0.34、0.68、1.35 g/kg），每组10只。除正常对照组外，其余各组大鼠均采用干姜水煎液结合5％乙醇给药复制血热出血模型。造模成功后，除正常对照组和模型组大鼠ig生理盐水外，其余各组大鼠ig相应药物，每天1次，连续14 d。末次给药1 h后检测各组大鼠体质量、摄食量、饮水量、血小板聚集率、组织型纤溶酶原激活剂（t-PA）和纤溶酶原激活物抑制剂（PAI-1）的含量；检查肺、胃组织病理学变化。结果：与正常对照组比较，模型组大鼠体质量、摄食量、血小板最大聚集率、PAI-1含量均减少，饮水量、t-PA含量及t-PA/PAI-1比值均增加（P＜0.05或P＜0.01），肺、胃组织均表现出出血和炎症等病理变化。与模型组比较，云南白药组和BSP低、中、高剂量组大鼠体质量、摄食量均增加，饮水量减少（P＜0.05或P＜0.01）；云南白药组和BSP中、高剂量组大鼠血小板最大聚集率、PAI-1含量均增加，t-PA/PAI-1比值减少（P＜0.05或P＜0.01），肺、胃组织的病理变化均不同程度减轻。结论：BSP可能通过升高血小板聚集率、调节纤溶系统、改善肺和胃组织的病变，综合发挥止血作用。
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