组织自发荧光在大鼠心肌缺血实验中的应用

摘要

Aim Todiscusstheapplicationoftissue spontaneous fluorescence in myocardial ischemia injury of rats based on specific fluorescence detection technol-ogy.Methods Thechangeofspontaneousfluores-cence was compared between the myocardial tissue of normal rats and those of rats with myocardial ischemia injury and an quantitative analysis was then made.Re-sult Theresultsshowedthatspontaneousfluorescence of myocardial tissue for myocardial ischemia injury changed significantly.Spontaneous fluorescence signal of injury considerably was enhanced.The fluorescence signal which was quantified by FX Pro had statistical significance compared with normal myocardial tissue,P<0.01orP<0.05.Conclusion Ourresearchshows that spontaneous fluorescence of myocardial tissue can be enhanced obviously after myocardial ischemia inju-ry.Our research provides a method for the research and evaluation of myocardial ischemia injury model in rats which can be used in positioning,qualitative and quan-titative comparative study and in pathological,physio-logical,pharmacological and mechanism study.%目的基于特定的荧光检测技术，探讨组织自发荧光在大鼠心肌缺血性损伤实验中的应用。方法本研究通过Kodak FX Pro活体成像技术检测大鼠心肌组织的自发荧光。通过荧光检测，比较正常大鼠和心肌缺血性损伤大鼠的心肌组织对应部位自发荧光的变化，并进行定量分析。结果心肌缺血损伤后，损伤部位组织自发荧光信号明显增强，将荧光信号通过活体成像系统进行定量，与正常心肌组织比较差异有统计学意义。结论大鼠心肌组织在心肌缺血损伤后，心肌组织的自发荧光明显增强，为大鼠心肌缺血损伤模型的研究及其评价提供新的方法。有利于定位、定性、定量比较研究，可用于病理、生理、药理及作用机制等多方面研究。