人参皂苷Re对H9 c2心肌细胞CYP450酶的影响

摘要

Aim To observe the cytochrome 450 effect of ginsenoside Re on H9c2 cells, in order to clarify the molecular mechanism of ginsenoside Re. Methods H 9 c 2 cells were separately treated with ginsenoside Re for 1, 5, 10, 50, 100 μmol·L-1 or 6, 24, 36, 48, 60 h. CYP2C11, 2J3, 4A1, 4A3, 4F4 and ANP mR-NA expressions were analyzed by Real time PCR, and CYP4 A1 , 2 J3 protein expressions were detected by Western blot. Results Compared with control group, ginsenoside Re could effectively upregulate CYP2 C11 , CYP2 J3 , ANP mRNA expression to 1. 6 , 1. 8 , 3. 2 fold, and downregulate CYP4A1, CYP4A3, CYP4F4 mRNA expression to 0. 4, 0. 15, 0. 3 fold. Ginsen-oside Re could decrease CYP4 A1 protein expression in a concentration-dependent manner, while ginsenoside Re could increase CYP2 J3 protein expression in a con-centration-dependent manner. Conclusion Ginsen-oside could regulate CYP450 enzyme and change ANP gene expression, which might be the molecular mecha-nism of ginsenoside Re.%目的：观察人参皂苷Re对H9c2心肌细胞色素P450酶的影响，旨在发现人参皂苷Re对心肌细胞作用的分子机制。方法实验分对照组、人参皂苷Re各剂量组(1、5、10、50、100μmol · L-1)处理组，人参皂苷Re作用6、24、36、48及60 h后提取RNA或蛋白进行测定。利用实时定量PCR法检测 H9c2心肌细胞 CYP2C11、 CYP2J3、 CYP4A1、CYP4A3、CYP4F4及 ANP mRNA 的表达；采用 Western blot法检测心肌细胞CYP4A1和CYP2J3蛋白的表达。结果人参皂苷Re明显上调心肌细胞CYP2C11、CYP2J3 mRNA的表达至正常对照的1．6、1．8倍，明显下调CYP4A1、CYP4A3及CYP4F4 mRNA的表达至正常对照的0．4、0．15、0．3倍。人参皂苷Re明显上调ANP基因表达水平至正常对照的3.2倍。随着药物浓度的增加，人参皂苷Re对CYP4A1蛋白表达产生明显的下调作用，而对CYP2J3蛋白产生明显的上调作用。结论人参皂苷Re可影响心肌细胞的CYP450酶和ANP基因的表达。