左旋龙脑对人脐静脉内皮细胞氯通道和细胞容积的影响

摘要

Aim To study the effects of L-borneol on the chloride channel and cell volume of human umbili-cal vein endothelial cells (HUVECs). Methods Whole-cell patch-clamp technique was used to record chloride currents. The expression of ClC-3 protein was down-regulated by siRNA interference technique. The cell volume was measured by dynamic image analysis. Results 20 nmol·L-1L-borneol significantly activa-ted chloride current in HUVEC (79.59 ± 4.90) pA/pF, which could be inhibited by chloride channel blockers,NPPB and DIDS. The outward current inhib-itory rate of NPPB was (95.57 ± 2.57)%, while that of DIDS was (97.28 ± 6.36)%. The chloride current activated by L-borneol significantly decreased after the silence of ClC-3 (27.03 ± 3.89) pA/pF. Cell volume was markedly reduced by L-borneol (14.38 ± 1.58)%,which was inhibited after NPPB appliance. Conclusion L-borneol can activate ClC-3 chloride channel in HUVECs, which induces Cl- outflow then cell volume decrease.%目的 研究左旋龙脑对人脐静脉内皮细胞株(human umbilical vein endothelial cells,HUVECs)氯通道和细胞容积的作用.方法 膜片钳技术记录全细胞氯电流;siRNA 干扰技术下调ClC-3表达;动态图像分析测量细胞容积.结果20 nmol·L-1左旋龙脑能明显激活HUVECs氯电流,电流密度达(79.59 ± 4.90)pA/pF,左旋龙脑激活的电流能被氯通道阻断剂NPPB、DIDS抑制,外向电流抑制率分别为(95.57 ± 2.57)%、(97.28 ± 6.36)%;下调ClC-3氯通道表达后,左旋龙脑激活的氯电流明显减小至(27.03 ± 3.89)pA/pF;左旋龙脑能明显诱导细胞容积缩小(14.38 ± 1.58)%,氯通道阻断剂NPPB阻断左旋龙脑诱导的细胞容积改变.结论左旋龙脑能激活HUVECs的ClC-3氯通道,使Cl-外流诱发细胞容积减小.