Aim To study the therapeutic effect of re-combinant human acidic fibroblast growth factor (rh-aFGF) carbomer 940 gel in the treatment of skin wound healing in type I diabetic rats. Methods Two types of skin trauma models, namely, full-thickness wound and scalded wound,were established in a model of type I diabetes mellitus using STZ-induced SD rats. The rats were divided into control group, vehicle group,90 AU rh-aFGF gel group and 270 AU rh-aFGF gel group in each skin wound models. The wound area and wound healing rate were used to evaluate the thera-peutic effect. The growth of fibroblasts, fibrocytes, collagen fibers and vessel capillaries in the wound was observed using HE staining and analysed by semi-quantitative score. Results The rh-aFGF carbomer gel significantly reduced the traumatic area as well as promoted the wound healing rate of the skin trauma model of SD rats of type I diabetes mellitus (P <0.05). HE staining showed that rh-aFGF carbomer gel significantly promoted the pathological score of fibro-blasts and collagen fibers(P<0.05). Conclusions rh-aFGF carbomer gel might play a protective role in micro-environment of wound and rh-aFGF, which could benefit for proliferation of fibroblasts and colla-gen, therefore promoting the healing process of skin wound in SD rats with type I diabetes mellitus, and it might be expected to be a new preparation for the treat-ment of chronic trauma in diabetes mellitus.%目的 研究重组人酸性成纤维细胞生长因子(recombi-nant human acidic fibroblast growth factor, rh-aFGF)卡波姆940凝胶对糖尿病大鼠皮肤创伤修复作用.方法 使用STZ诱导产生SD大鼠糖尿病模型,建立全层皮肤切除和烫伤两种皮肤创伤模型,设置对照组、赋形剂组、90 AU rh-aFGF凝胶组和270 AU rh-aFGF凝胶组4个实验组.用创伤面积和愈合速率评价各组疗效;HE染色观察创面中成纤维细胞、肌成纤维细胞、胶原纤维和毛细血管生长情况;半定量评分评价rh-aFGF诱导的上述指标生长情况.结果 rh-aFGF卡波姆940凝胶明显减少糖尿病SD大鼠全层皮肤切除和烫伤模型的皮肤损伤面积(P<0.05),促进其创伤愈合速率(P<0.05).HE染色结果表明,rh-aFGF卡波姆凝胶明显促进成纤维细胞和胶原纤维增殖评分(P<0.05).结论 rh-aFGF卡波姆凝胶可能在一定程度上保护创面微环境和保护 rh-aFGF发挥生物学活性,促进成纤维细胞增殖和胶原纤维沉积,从而促进糖尿病SD大鼠皮肤创伤愈合过程,有望成为一种治疗糖尿病溃疡的新制剂.
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