神经肽P物质对哮喘大鼠气道阻力的影响

摘要

Objective To investigate the effect of neuropeptide substance P on airway resiatance in rats with asthma.Methods Sixty Wistar rats were randomly divided into 3 groups including normal control group(n =20),asthma group(n =20) and substance P group(n =20).Asthmatic rat model was established.We detected inspiratory resistance and expiratory resistance in the airway of rats in each group with the intervention of different concentrations (0.062 5,0.125 0,0.250 0,0.5000 mg/ml) of methacholine(MCh) by using animal pulmonary function analyzer.Substance P group rats were given substance P ( 10-7 mol/L,0.2 ml) when airway resistance was detected.Results With the increase in MCh concentration,the increase in airway resistance in rats was found,rat airway inspiratory resistance and expiratory resistance were all positively correlated with the concentration of MCh ( r=0.947,P＜0.05 ; r=0.965,P＜0.05 ).With the intervention of different concentration of Mch (0.0625,0.125 0,0.2500,0.5000 mg/ml),the significant differences were found among the three groups in rat airway inspiratory resistance ( F=73.4,89.3,91.2,106.4,P＜0.05) and expiratory resistance(F=67.3,77.5,83.4,126.5,P＜0.05).Airway resistance to MCh concentration response curves were shifted upward significantly in asthma group compared with normal control group and in substance P group compared with asthma group.Inspiratory resistance and expiratory resistancc in asthma group were significantly increased compared with normal control group( P＜0.05)and in sutstance P group compared with asthma group( P＜0.05 ).Conclusion Substance P can increase asthmatic rat's airway resistance and the airway resistance increase with the increasing of MCh concentration.%目的 探讨神经肽P物质对哮喘大鼠气道阻力的影响.方法 按照随机分组的原则将60只Wistar大鼠分为正常对照组、哮喘组及P物质干预组,每组各20只.制作哮喘大鼠模型.应用动物肺功能分析仪检测各组大鼠在浓度为0.0625、0.1250、0.250 0、0.5000 mg/ml乙酰甲胆碱(methacholine,MCh)干预下的气道吸气阻力及呼气阻力,其中P物质干预组大鼠在测定气道阻力时于气道内滴入0.2ml浓度为10-7 mol/L的P物质.结果 随着MCh浓度增加,大鼠气道阻力增加,大鼠气道吸气阻力及呼气阻力均与MCh浓度呈正相关(r=0.947,P＜0.05;r=0.965,P＜0.05).在0.0625、0.125 0、0.2500、0.5000 mg/ml浓度梯度的MCh作用下,3组间大鼠气道吸气阻力比较差异具有统计学意义(F值分别为73.4、89.3、91.2、106.4,P＜0.05)；呼气阻力比较差异具有统计学意义(F值分别为67.3、77.5、83.4、126.5,P＜0.05).哮喘组大鼠气道阻力对MCh的浓度反应曲线较正常对照组明显上移,其吸气阻力和呼气阻力在不同浓度MCh作用下均高于正常对照组,差异有统计学意义(P＜0.05)；P物质干预组大鼠对MCh的浓度反应曲线较哮喘组明显上移,在不同浓度MCh刺激下吸气阻力和呼气阻力均高于哮喘组,差异有统计学意义(P＜0.05).结论 神经肽P物质可增加哮喘大鼠的气道阻力,并随着MCh浓度增加气道阻力增加.